| 引用本文: | 汤秋华,林辉,张一帆,黄祥昌.双波长HPLC同时测定复方红花喷雾剂中羟基红花黄色素A与绿原酸的含量[J].中国现代应用药学,2016,33(3):325-328. |
| TANG Qiuhua,LIN Hui,ZHANG Yifan,HUANG Xiangchang.Simultaneous Determination of Hydroxysaffor Yellow A and Chlorogenic Acid in Fufang Honghua Spray by Dual-wavelength HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(3):325-328. |
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| 摘要: |
| 目的 建立同时测定复方红花喷雾剂中羟基红花黄色素A(hydroxysafflor yellow A，HSYA)与绿原酸含量的方法。方法 采用双波长HPLC，色谱柱：Shim-pack VP-ODS(250 mm×4.6 mm，5 μm)，流动相：乙腈-0.4%磷酸溶液(12∶88)，流速为1.0 mL·min-1，检测波长：403 nm(HSYA)和327 nm(绿原酸)，柱温为30 ℃。结果 HSYA、绿原酸的浓度分别在0.612~12.24，0.632~12.64 μg·mL-1内与各自峰面积呈良好的线性关系(r均为0.999 9)；平均加样回收率分别为98.34%和99.82%，RSD分别为1.16%和1.64%(n=9)。结论 该方法操作简便、快速，结果可靠，具有良好的重复性，可用于复方红花喷雾剂中HSYA与绿原酸的含量测定。 |
| 关键词: 复方红花喷雾剂 羟基红花黄色素A 绿原酸 双波长高效液相色谱法 |
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| 基金项目:福建省泉州市科技计划项目(2013Z76) |
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| Simultaneous Determination of Hydroxysaffor Yellow A and Chlorogenic Acid in Fufang Honghua Spray by Dual-wavelength HPLC |
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TANG Qiuhua, LIN Hui, ZHANG Yifan, HUANG Xiangchang
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Quanzhou First Hospital Affiliated to Fujian Medical College, Quanzhou 362000, China
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| Abstract: |
| OBJECTIVE To establish a method for the simultaneous determination of hydroxysaffor yellow A(HSYA) and chlorogenic acid in Fufang Honghua spray. METHODS The dual-wavelength HPLC was adopted. The Shim-pack VP-ODS column(250 mm×4.6 mm, 5 μm) was used with mobile phase of acetonitrile-0.4% phosphonic acid(12∶88) at flow rate of 1.0 mL·min-1, and the detection wavelength was set at 403 nm for HSYA and 327 nm for chlorogenic acid. The column temperature was 30 ℃. RESULTS The method displayed good linearity over the concentration ranges of 0.612-12.24 μg·mL-1 for HSYA and 0.632-12.64 μg·mL-1 for chlorogenic acid(r=0.999 9), respectively. The average recoveries were 98.34% and 99.82%, with the RSD of 1.16% and 1.64% (n=9), respectively. CONCLUSION This method is simple, rapid, reliable and reproducible. It can be used for determination of HSYA and chlorogenic acid in Fufang Honghua spray. |
| Key words: Fufang Honghua spray hydroxysaffor yellow A chlorogenic acid dual-wavelength HPLC |
