| 引用本文: | 王玮,朱声挥,郭闻渊,滕飞,位华,陈万生.LC-MS/MS测定肝移植患者体内他克莫司和五酯胶囊主要成分的方法学建立及应用[J].中国现代应用药学,2016,33(7):854-859. |
| WANG Wei,ZHU Shenghui,GUO Wenyuan,TENG Fei,WEI Hua,CHEN Wansheng.Simultaneous Determination of Tacrolimus and Five Main Compounds of Wuzhi Capsule in Liver Transplant Patients’ Whole Blood by LC-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(7):854-859. |
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| 摘要: |
| 目的 建立应用LC-MS/MS同时测定移植患者体内他克莫司和五酯胶囊主要成分的方法，并应用所建立的方法研究肝移植患者体内各待测物的药动学特征。方法 患者全血样本经前处理后进样，应用LC-MS/MS检测他克莫司、五味子甲素、五味子酯甲、五味子醇甲、五味子醇乙、五味子酚的浓度，同时进行方法学验证。应用所建立的方法，测定肝移植患者服用他克莫司和五酯胶囊后多个时间点的血药浓度，研究其主要成分的药动学过程。结果 患者全血样本中6种待测物专属性好，在各自线性范围内标准曲线符合要求，日内、日间精密度均<15%，提取回收率与基质效应范围在80%~120%，样本稳定性好。结论 该方法专属性强、灵敏度高、快速准确，适用于移植患者体内他克莫司、五味子甲素、五味子酯甲、五味子醇甲、五味子醇乙、五味子酚的血药浓度分析与测定。 |
| 关键词: 他克莫司 五酯胶囊 LC-MS/MS 肝移植患者 药动学 |
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| 基金项目:国家自然科学基金青年基金项目(81303113)；上海市卫生局中医药科研基金课题(12401906300) |
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| Simultaneous Determination of Tacrolimus and Five Main Compounds of Wuzhi Capsule in Liver Transplant Patients’ Whole Blood by LC-MS/MS |
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WANG Wei1, ZHU Shenghui2, GUO Wenyuan3, TENG Fei3, WEI Hua1, CHEN Wansheng1
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1.Department of Pharmacy, Shanghai Changzheng Hospital, Shanghai 200001, China;2.Department of Laboratory Test, Shanghai Changzheng Hospital, Shanghai 200001, China;3.Department of Organ Transplantation, Shanghai Changzheng Hospital, Shanghai 200001, China
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| Abstract: |
| OBJECTIVE To establish a LC-MS/MS analytical method for simultaneous determination of tacrolimus and five compounds of Wuzhi capsule in liver transplant recipients’ whole blood and to study the pharmacokinetic characteristic of the analytes in liver transplant patients. METHODS The zinc sulfate was added to whole blood to lyse the blood cells, and then the sample was deposited by methanol. The upper layer was injected into the LC-MS/MS. Then a LC-MS/MS method was established for the determination of tacrolimus, schisandrin, schisandrol B, schisantherin A, deoxyshisandrin and schisanhenol. RESULTS The calibration curve of six compounds showed a good linear relationship. The intra and inter day precisions and accuracy met the requirements and the extraction recovery was all right. CONCLUSION This method is accurate, precise, sensitive and specific to be suitable for concentration of therapeutic drug monitoring and pharmacokinetic study of tacrolimus, schisandrin, schisandrol B, schisantherin A, deoxyshisandrin and schisanhenol. |
| Key words: tacrolimus Wuzhi capsule LC-MS/MS liver transplant patients pharmacokinetics |
