| 引用本文: | 张秀芝,苗浩,王济兴,吕博杰,陈建双.辛伐他汀联合辅酶Q10对慢性心衰大鼠心肌转化生长因子β1表达的影响[J].中国现代应用药学,2016,33(3):290-293. |
| ZHANG Xiuzhi,MIAO Hao,WANG Jixing,LYU Bojie,CHEN Jianshuang.Effect of Simvastatin and Coenzyme Q10 on Expression of TGF-β1 in Rats with Chronic Heart Failure[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(3):290-293. |
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| 摘要: |
| 目的 探讨辛伐他汀联合辅酶Q10对慢性心力衰竭大鼠心肌转化生长因子β1(transforming growth factor-β1，TGF-β1)表达的影响。方法 利用腹主动脉缩窄法制作SD大鼠慢性心力衰竭模型，随机分为模型组、辛伐他汀组、辅酶Q10组、辛伐他汀+辅酶Q10组，另设假手术组，每组8只大鼠，观察各组大鼠血流动力学参数(左室舒张末压、±dp/dtmax) 和左心室心肌组织病理学改变，SP免疫组化检测各组大鼠左心室心肌中TGF-β1表达情况。结果 与模型组相比，辛伐他汀组、辅酶Q10组和辛伐他汀+辅酶Q10组左室舒张末压显著降低(P<0.01)，±dp/dtmax显著升高(P<0.01)，左心室肌TGF-β1阳性表达水平显著降低(P<0.01)；联合用药较辛伐他汀组、辅酶Q10改变更为显著(P<0.05)。结论 辛伐他汀与辅酶Q10联合应用较两药单独应用改善心衰大鼠心功能、抑制心室重塑和减少TGF-β1表达作用明显。 |
| 关键词: 辛伐他汀 辅酶Q10 慢性心力衰竭 转化生长因子β1 |
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| 基金项目:河北省卫生厅医学科学研究重点课题计划项目(20110180) |
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| Effect of Simvastatin and Coenzyme Q10 on Expression of TGF-β1 in Rats with Chronic Heart Failure |
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ZHANG Xiuzhi1, MIAO Hao2, WANG Jixing3, LYU Bojie3, CHEN Jianshuang2
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1.Xinglong Maternal and Child Health Hospital, Chengde 067000, China;2.Chengde Medical College, Chengde 067000, China;3.PBL NO.266 Hospital, Chengde 067000, China
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| Abstract: |
| OBJECTIVE To study the effect of simvastatin and coenzyme Q10 on expression of transforming growth factor-β1(TGF-β1) in rats with chronic heart failure(CHF). METHODS SD rats were randomly divided into 5 groups: model group, simvastatin group, coenzyme Q10, simvastatin combined coenzyme Q10 group and sham-operated group. The CHF model was established with coarctation of abdominal aorta(CCA). Hemodynamic of left ventricular end-diastolic diameter press(LVEDP), left ventricular pressure rise and fall (±dp/dtmax) was tested; HE and Masson’s trichrome staining was carried out for morphology. Expression of TGF-β1 in left ventricle was assayed by SP immunohistochemistry. RESULTS Compared with model group, LVEDP in other groups was significantly lower(P<0.01), ±dp/dtmax significantly higher(P<0.01); in addition, protein expression of TGF-β1 in left ventricle tissue was also reduced, while simvastatin combined coenzyme Q10 had more obvious effects on improving heart fouction of rats with cardiac remodeling and decreasing expression of TGF-β1 protein. CONCLUSION Simvastatin combined with coenzyme Q10 simvastatin can enhance the efficacy of single treatment on heart fouction, and protein expression of TGF-β1. |
| Key words: simvastatin coenzyme Q10 chronic heart failure TGF-β1 |
