| 引用本文: | 张洁红,金银秀,王玉新,施秋婷,沈佩佩,夏念友,黄雅静,赵均炼.苯扎贝特纳米脂质载体的包封率测定[J].中国现代应用药学,2016,33(4):433-436. |
| ZHANG Jiehong,JIN Yinxiu,WANG Yuxin,SHI Qiuting,SHEN Peipei,XIA Nianyou,HUANG Yajing,ZHAO Junlian.Determination of Entrapment Efficiency of Bezafibrate Nanostructured Lipid Carriers[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(4):433-436. |
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| 摘要: |
| 目的 建立苯扎贝特纳米脂质载体包封率的测定方法。方法 采用葡聚糖凝胶柱层析法分离苯扎贝特纳米脂质载体中的游离药物，高效液相色谱法测定其含量，计算包封率。色谱条件：采用Agilent ZORBAX SB-C18柱(250 mm×4.6 mm，5 μm)，流动相为0.01 mol·L-1磷酸盐缓冲液(pH 3.0)-甲醇(30∶70)，流速：1.0 mL·min-1，检测波长：230 nm，进样量：20 μL。结果 苯扎贝特峰与杂质峰的分离度良好，苯扎贝特浓度在4.44~177.6 μg·mL-1内与峰面积呈良好的线性关系(r=0.999 9)，平均回收率为99.23%(RSD=0.68%)。3批苯扎贝特纳米脂质载体的包封率均>75%。结论 本方法简便、准确，灵敏度高，可用于苯扎贝特纳米脂质载体的质量控制。 |
| 关键词: 苯扎贝特 纳米脂质载体 包封率 葡聚糖凝胶柱层析法 高效液相色谱法 |
| DOI： |
| 分类号:R917.101 |
| 基金项目:台州市大学生科技创新项目；台州市科技计划项目(1402ky18)；浙江省教育厅一般科研项目(Y201432771、Y201533944)；台州市创新团队和重点实验室自主设计科技计划项目(1403ky12) |
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| Determination of Entrapment Efficiency of Bezafibrate Nanostructured Lipid Carriers |
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ZHANG Jiehong, JIN Yinxiu, WANG Yuxin, SHI Qiuting, SHEN Peipei, XIA Nianyou, HUANG Yajing, ZHAO Junlian
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Chemical Pharmaceutical Research Institute, Taizhou Vocational Technical College, Taizhou 318000, China
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| Abstract: |
| OBJECTIVE To establish a method for the determination of entrapment efficiency of bezafibrate nanostructured lipid carriers. METHODS Free bezafibrate was separated from the nanostructured lipid carriers by Sephadex gel, with HPLC on analysis for determination of entrapment efficiency of bezafibrate nanostructured lipid carriers. An Agilent ZORBAX SB-C18 column(250 mm×4.6 mm, 5 μm) was used, and 0.01 mol·L-1 phosphate buffer (pH 3.0)-methanol(30∶70) was used as the mobile phase. The flow rate was 1.0 mL·min-1. The detection wavelength was 230 nm. The sample volume was 20 μL. RESULTS The peak of bezafibrate and impurity could be separated well. The linear range of bezafibrate was 4.44-177.6 μg·mL-1(r=0.999 9). The recovery of bezafibrate was 99.23%(RSD=0.68%). The entrapment efficiency of 3 batches of bezafibrate nanostructured lipid carriers were all >75%. CONCLUSION The proposed method is simple, applicable and accurate for determination of entrapment efficiency of bezafibrate nanostructured lipid carriers. |
| Key words: bezafibrate nanostructured lipid carriers entrapment efficiency Sephadex gel column chromatography HPLC |
