| 引用本文: | 魏伟,李博乐,王晨.包裹5-氟尿嘧啶的二氧化硅纳米颗粒的制备及细胞毒性研究[J].中国现代应用药学,2016,33(1):59-62. |
| WEI Wei,LI Bole,WANG Chen.Synthesis of 5-Fluorouracil Doped Silica Nanoparticles and Their Cytotoxicity[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(1):59-62. |
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| 摘要: |
| 目的 制备包裹5-氟尿嘧啶的二氧化硅(5-Fu/SiO2)纳米颗粒，并对其药剂学参数及体外细胞毒性进行研究。方法 利用反相微乳化法制备5-Fu/SiO2纳米颗粒，对5-Fu投入量及反应时间等合成条件进行优化，考察纳米颗粒的稳定性及体外释药行为，并采用MTT法对其体外细胞毒性进行研究。结果 当5-Fu投入量为1.33 mg及反应时间为24 h时，5-Fu/SiO2纳米的载药率及包封率达到最高，分别为1.03%及24.77%，同时该纳米在48 h内释放率达到41.31%，7 d内粒径无明显变化。细胞毒性实验表明，5-Fu/SiO2纳米对人肝癌细胞具有明显的抑制效果，而空白SiO2纳米对细胞活性影响较小。结论 成功制备了稳定性好、缓释时间长的5-Fu/SiO2纳米颗粒，为开发5-Fu缓释剂型提供了新的思路。 |
| 关键词: 5-氟尿嘧啶 二氧化硅纳米 细胞毒性；药物载体 |
| DOI： |
| 分类号:R284.1；R917.101 |
| 基金项目:天津医科大学肿瘤医院药检放专项基金(Y1208) |
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| Synthesis of 5-Fluorouracil Doped Silica Nanoparticles and Their Cytotoxicity |
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WEI Wei, LI Bole, WANG Chen
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Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Department of Pharmacy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China
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| Abstract: |
| OBJECTIVE To synthesize 5-fluorouracil(5-Fu) doped silica(5-Fu/SiO2) nanoparticles, and to evaluate their parameter of pharmacy and cytotoxicity in vitro. METHODS The 5-Fu/SiO2 nanoparticles were prepared in the water-in-oil microemulsion, and the administered dose of 5-Fu and the reaction time were optimized to improve the drug loading and entrapment efficiency. The stability and the in vivo release of 5-Fu/SiO2 were also investigated. The antitumor activities in vitro were evaluated by MTT method. RESULTS With adding 1.33 mg 5-Fu and reacting 24 h, the 5-Fu loading and entrapment efficiency reached maximum yields as 1.03% and 24.77%, respectively. The 5-Fu was released slowly for 48 h, and the size of 5-Fu/SiO2 nanoparticles had no obvious changing in 7 d. The MTT experiments showed that 5-Fu/SiO2 nanoparticles had an inhibition on the human hepatic cancer cells, while the SiO2 nanoparticles had no similar effects. CONCLUSION The 5-Fu/SiO2 nanoparticles with a good stability and long release is prepared successfully, which provides a new fitting way for developing the 5-Fu dosage forms. |
| Key words: 5-fluorouracil silica nanoparticles cytotoxicity pharmaceutical carrier |
