| 引用本文: | 周燕,薛磊冰,金佩芬.枳壳及其制剂血府逐瘀胶囊中柚皮苷和新橙皮苷的含量测定[J].中国现代应用药学,2016,33(6):778-780. |
| ZHOU Yan,XUE Leibing,JIN Peifen.Determination of Naringin and Neohesperidin in Aurantii Fructus and its Preparation Xuefu Zhuyu Capsules[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(6):778-780. |
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| 摘要: |
| 目的 建立HPLC同时测定枳壳和血府逐瘀胶囊中柚皮苷和新橙皮苷的含量。方法 色谱柱为SunFireTM C18(250 mm×4.6 mm，5 μm)；流动相为乙腈-水(20∶80)，磷酸调pH至2.5；流速为1.0 mL·min-1；检测波长为283 nm。结果 柚皮苷在0.166~2.074 μg内线性关系良好(r=0.999 8)，平均加样回收率为98.17%；新橙皮苷在0.162~2.020 μg内线性关系良好(r=0.999 5)，平均加样回收率为100.61%。结论 本方法简便、准确，重复性好，可用于枳壳及其制剂血府逐瘀胶囊中柚皮苷和新橙皮苷的含量测定。 |
| 关键词: 枳壳 血府逐瘀胶囊 柚皮苷 新橙皮苷 高效液相色谱法 |
| DOI： |
| 分类号:R284.1；R917.101 |
| 基金项目: |
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| Determination of Naringin and Neohesperidin in Aurantii Fructus and its Preparation Xuefu Zhuyu Capsules |
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ZHOU Yan, XUE Leibing, JIN Peifen
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Jiaxing Drug and Food Vocational College, Jiaxiang 314001, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the determination of naringin and neohesperidin in Aurantii Fructus and its preparation Xuefu Zhuyu Capsules. METHODS The column was SunFireTM C18(250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water(20∶80) adjusted to pH 2.5 with phosphoric acid. The flow rate was 1.0 mL·min-1 and the detection wavelength was at 283 nm. RESULTS The calibration curves were linear within the range of 0.166-2.074 μg(r=0.999 8) for naringin, 0.162-2.020 μg(r=0.999 5) for neohesperidin, respectively. The average recovery of them were 98.17% and 100.61%, respectively. CONCLUSION The method is simple, practicable, accurate and rapid. It can be applied to content determination of naringin and neohesperidin in Aurantii Fructus and Xuefu Zhuyu Capsules. |
| Key words: Aurantii Fructus Xuefu Zhuyu Capsules naringin neohesperidin HPLC |
