芍药甘草汤对哮喘大鼠Treg/Th17失衡及其相关细胞因子的干预作用

徐俭朴; 何飞; 杨明华; 汝触会; 吴滨; 蔡宛如<sup>*</sup>

引用本文:	徐俭朴,何飞,杨明华,汝触会,吴滨,蔡宛如.芍药甘草汤对哮喘大鼠Treg/Th17失衡及其相关细胞因子的干预作用[J].中国现代应用药学,2016,33(1):19-23.
	XU Jianpu,HE Fei,YANG Minhua,RU Chuhui,WU Bin,CAI Wanru.Intervention Effect of Shaoyao Gancao Decoction on the Imbalance of Treg/Th17 and Their Related Cytokine in Rats with Asthma[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(1):19-23.

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芍药甘草汤对哮喘大鼠Treg/Th17失衡及其相关细胞因子的干预作用
徐俭朴¹, 何飞¹, 杨明华², 汝触会¹, 吴滨³, 蔡宛如⁴
1.浙江中医药大学附属中西医结合医院，杭州 310003;2.浙江省中药研究所，杭州 310023;3.浙江萧山医院，杭州 311202;4.浙江中医药大学附属第二医院，杭州 310005

摘要:

目的观察芍药甘草汤对支气管哮喘SD大鼠Treg/Th17失衡及相关细胞因子IL-2、IL-6、IL-10、IL-17的干预情况，探讨芍药甘草汤防治哮喘的作用机制。方法 SD大鼠40只，♂，随机均分为正常对照组、模型组、芍药甘草汤组、地塞米松组、中西医结合组，每组8只。除正常对照组外其余各组大鼠以卵白蛋白致敏并诱发哮喘模型。正常对照组和模型组生理盐水灌胃，其余组给予对应药物，用Elisa酶联免疫法测定肺匀浆中相关细胞因子IL-2、IL-6、IL-10、IL-17水平。流式细胞仪检测大鼠脾脏CD4⁺细胞数及比例。结果与模型组比较，各药物组均可显著降低IL-2、IL-6及IL-17水平(P<0.01)，同时能显著升高IL-10含量(P<0.01)；各药物组之间比较IL-2、IL-6和IL-10差异无统计学意义(P>0.05)；地塞米松组IL-17表达比芍药甘草汤组及中西医结合组均显著降低(P<0.05)。与模型组比较，芍药甘草汤组能显著增加CD4⁺CD25⁺Foxp3⁺Treg细胞的比例(P<0.01)。结论芍药甘草汤防治哮喘的作用机制可能与调节哮喘大鼠Treg/Th17比例及其相关细胞因子表达的作用有关。

关键词: 芍药甘草汤哮喘大鼠 Treg/Th17细胞

DOI：

分类号:

基金项目:国家自然科学基金(81403336)；杭州市科技发展计划项目(20120533Q27)

Intervention Effect of Shaoyao Gancao Decoction on the Imbalance of Treg/Th17 and Their Related Cytokine in Rats with Asthma

XU Jianpu¹, HE Fei¹, YANG Minhua², RU Chuhui¹, WU Bin³, CAI Wanru⁴

1.Integrated Chinese and Western Medicine Hospital of Zhejiang Province, Hangzhou 310003, China;2.Zhejiang Provincial Research Institute of Chinese Materia Medic, Hangzhou 310023, China;3.Zhejiang Xiaoshan Hospital, Hangzhou 311202, China;4.The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310005, China

Abstract:

OBJECTIVE To observe the effects of Shaoyao Gancao decoction on the imbalance of Treg/Th17 and their related cytokine in rats with asthma, and to explore its mechanism in treating asthma. METHODS SD rats, ♂, were randomly divided into normal control group, model group, Shaoyao Gancao decoction group, dexamethasone group and integrated TCM-WM group(n=8 for each group). Asthma was induced by intraperitoneal injection of ovalbumin (OVA) and forced inhalation of atomized OVA. Expression of IL-2, IL-6, IL-10 and IL-17 was detected by Elisa method. Numbers of CD4⁺ T cells in spleen was analyzed by flow cytometry. RESULTS Compared with model group, expression of IL-6, IL-17 and IL-2 in lung homogenate were decreased significantly in the treated group(P<0.01) and expressions of IL-10 was significantly increased in the treated groups(P<0.01). There were no significant differences of expression of IL-2, IL-6 and IL-10 among the treated groups(P>0.05). Expression of IL-17 was decreased significantly in the dexamethasone group than other treated groups(P<0.05). Compared with model group, Shaoyao Gancao decoction increased the percentages of CD4⁺CD25⁺Foxp3⁺ regulatory T cell(P<0.01). CONCLUSION Shaoyao Gancao decoction can efficiently regulate the imbalance of Treg/Th17 and their related cytokine, which may be one of its mechanisms in preventing and treating asthma.

Key words: Shaoyao Gancao decoction rats with asthma Treg/Th17 cell