| 引用本文: | 张虎,朱蕾.蛇床子素通过p53信号通路诱导人乳腺癌细胞株MCF-7凋亡[J].中国现代应用药学,2016,33(5):547-551. |
| ZHANG Hu,ZHU Lei.Osthole Induce Human Breast Cancer Cells Lines MCF-7 Apoptosis by Activating p53 Signaling Pathway[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(5):547-551. |
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| 摘要: |
| 目的 探讨蛇床子素对人乳腺癌细胞株MCF-7的作用及其机制。方法 对数生长期的MCF-7 细胞用0，25，50，100 mmol·L-1的蛇床子素处理。细胞培养48 h后，利用MTT检测细胞增殖；流式检测细胞凋亡和膜电位。RT-PCR检测p53、p21、BCL-2、Bax和CytC mRNA水平；免疫印迹法检测p53、p21、BCL-2、Bax 和CytC 表达。结果 蛇床子素可以成浓度依赖性诱导MCF-7 细胞增殖抑制和凋亡，上调Bax、p53、p21和CytC表达，下调 Bcl-2的表达以及细胞膜电位。结论 蛇床子素可通过激活p53信号通路而诱导MCF-7细胞凋亡。 |
| 关键词: 蛇床子素 MCF-7 增殖 凋亡 p53 |
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| Osthole Induce Human Breast Cancer Cells Lines MCF-7 Apoptosis by Activating p53 Signaling Pathway |
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ZHANG Hu, ZHU Lei
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Department of General Surgery, China Three Gorges University, Yichang 443000, China
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| Abstract: |
| OBJECTIVE To investigate the effect and mechanisms of the osthole on the human breast cancer cells lines MCF-7. METHODS The human breast cancer cells lines MCF-7 were cultured in vitro and the MCF-7 cell at logarithmic growth phase was treated with different concentration of osthole(0, 25, 50, 100 mmol·L-1) for 48 h. MTT and flow cytometric analysis were used to evaluate the proliferation, apoptosis, and membrane potential of MCF-7 cells. RT-PCR was used to examnine the p53, p21, BCL-2, Bax and CytC mRNA level. The expression of p53, p21, BCL-2, Bax and CytC in MCF-7 cell were detected by Western blotting. RESULTS Osthole treatment could induce the proliferation inhibition, apoptosis and the expression of Bax, p53, p21 and CytC with decreasing the membrane potential and the expression of BCL-2 on MCF-7 cell in a concentration dependent manner. CONCLUSION Osthole can induce the apoptosis of MCF-7 by activating p53 signaling. |
| Key words: osthole MCF-7 proliferation apoptosis p53 |
