| 引用本文: | 李文斌,张晓惠,王荣.直接进样液相色谱法在生物样品分析中的应用[J].中国现代应用药学,2016,33(1):122-127. |
| LI Wenbin,ZHANG Xiaohui,WANG Rong.Application of Liquid Chromatography with Direct Injection in the Analysis of Biological Samples[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(1):122-127. |
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| 摘要: |
| 生物样品基质复杂，在色谱分析前先要除去其中的蛋白质等大分子物质，以免损害色谱柱。常用的前处理方法多为离线操作，耗时费力，还会造成样品损失和污染，而直接进样的方法能减少前处理步骤，并能有效预浓缩和净化生物样品。这一过程可以通过柱切换技术实现自动化，尽可能地减少了生物样品的前处理步骤和潜在的样品污染，提高方法的重现性。本文对目前生物样品的液相色谱分析方法中样品自动化处理的进展进行了综述。具体集中于直接进样技术，如限进性填料和其他自动化在线固相萃取过程，还包括新兴的对样品更具选择性的自动化萃取相技术，如分子印迹聚合物。并对这些方法和潜在的应用前景进行了简要讨论。 |
| 关键词: 生物样品分析 直接进样 限进性填料 在线固相萃取 |
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| Application of Liquid Chromatography with Direct Injection in the Analysis of Biological Samples |
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LI Wenbin, ZHANG Xiaohui, WANG Rong
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Key Laboratory of the Plateau of the Environmental Damage Control, Lanzhou General Hospital of Lanzhou Military Command, Lanzhou 730050, China
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| Abstract: |
| The composition of biological samples is complicated, the protein and other macromolecules should be removed at first before analysis to avoid damaging the chromatographic column. The common methods used for pre-treatment are multi-line operation and time-consuming, furthermore cause damage and contamination of the sample. The method of direct injection can reduce the sample pretreatment step, effectively purify and pre-concentrate the sample. This process can be automated by column-switching technique, it will improve the reproducibility of the method. In this paper, automated processing of current progress of sample in liquid chromatography analysis are reviewed, especially focus on the direct injection technologies such as restricted access material and other automated online solid phase extraction process, also include the more selective emerging automated sample extraction phase techniques, such as molecular imprinted polymers. Finally, these methods and potential applications are briefly discussed. |
| Key words: biological sample analysis direct injection restricted access material online solid phase extraction |
