| 引用本文: | 甘盛,赖青鸟,李志成,韩婷,吴超权.表面增强拉曼光谱法对3种β受体激动剂化合物的定性和定量检测[J].中国现代应用药学,2015,32(11):1354-1359. |
| GAN Sheng,LAI Qingniao,LI Zhicheng,HAN Ting,WU Chaoquan.Qualitative and Quantitative Determination of 3 Kinds of β Agonist Molecules by Surface Enhanced Raman Spectrometry[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(11):1354-1359. |
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| 摘要: |
| 目的 以硫酸特布他林、盐酸班布特罗与盐酸氯丙那林为例，研究并建立利用表面增强拉曼光谱检测β受体激动剂的定性与定量方法。方法 选择800~850，1 050，1 450 cm-1处的3个特征峰作为快速鉴定样品中β受体激动剂类药物的定性依据，并选择盐酸氯丙那林为代表，在800~850 cm-1信号处进行定量检测。结果 盐酸氯丙那林在1~9 mg·L-1内线性关系良好，相关系数为R2=0.997 2，最低检出限为0.1 mg·L-1。结论 本法迅速简便，可实现无损检测，确证性高。 |
| 关键词: 表面增强拉曼光谱法 β受体激动剂 银溶胶 纳米颗粒 快速检测 |
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| 基金项目:中国食品药品检定研究院“中青年发展研究基金”项目(2013WC3)；“广西自然科学青年基金”项目(2014GXNSFBA118059) |
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| Qualitative and Quantitative Determination of 3 Kinds of β Agonist Molecules by Surface Enhanced Raman Spectrometry |
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GAN Sheng1, LAI Qingniao1, LI Zhicheng2, HAN Ting3, WU Chaoquan1
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1.Guangxi Institute for Food and Drug Control, Nanning 530021, China;2.Jinan Fruit Research Institute, All China Federation of Supply and Marketing Cooperatives, Jinan 250014, China;3.Ditactic and Research Section, Preclinical College, North University of Science and Technology, Tangshan 053009, China
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| Abstract: |
| OBJECTIVE To study and establish the method for determination of β agonists in human urine by surface enhanced Raman spectrometry, taking clorprenaline hydrochloride, bambuterol hydrochloride and clorprenaline hydrochloride as exemplars. METHORDS The peaks at 800-850, 1 050 and 1 450 cm-1 were identified as β agonist characteristics, while the assay was carried out taking clorprenaline hydrochloride at the peak 800-850 cm-1. RESULTS The calibration curve of dorprenaline hydrochloride was linear in the range of 1-9 mg·L-1, with the correlative coefficient R2=0.997 2. The limit of detection was 0.1 mg·L-1. CONCLUSION The method is proved to be rapid, simple, non-invasive and authentic. |
| Key words: surface enhanced Raman spectrometry β agonist silver sol nanoparticle screening |
