| 引用本文: | 王晓丹,夏晓玲,赵玉娇,王新华,张荣平,孙强明.蒲公英有机萃取物的抗甲型H1N1流感病毒作用[J].中国现代应用药学,2015,32(12):1423-1427. |
| WANG Xiaodan,XIA Xiaoling,ZHAO Yujiao,WANG Xinhua,ZHANG Rongping,SUN Qiangming.Anti-influenza A (H1N1) Virus Effect of Organic Solvent Extracts from Taraxaci Herba[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(12):1423-1427. |
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| 摘要: |
| 目的 通过检测蒲公英不同的萃取物体外抗甲型H1N1流感病毒作用，探讨其在甲型H1N1流感治疗上的应用。方法 首先用乙醇萃取而得到蒲公英的乙醇萃取物，然后依次采用乙酸乙酯、正丁醇、石油醚、水萃取蒲公英的乙醇萃取物，得到不同溶剂的萃取物；选用狗肾传代MDCK细胞作为病毒的宿主对病毒进行培养和扩增；通过血凝效价和Real time RT-PCR实验，间接或直接检测蒲公英不同萃取物对病毒的中和作用和增殖抑制作用。结果 4种不同溶剂的蒲公英萃取物作用病毒后，只有石油醚、乙酸乙酯组的血凝效价数值出现了一定程度的下降，其中乙酸乙酯萃取物组的值下降了2~4倍；而石油醚萃取物在96 h时，中和实验组和增殖抑制组的血凝效价值分别下降了256倍和128倍。Real time RT-PCR检测表明，石油醚和乙酸乙酯萃取物明显抑制病毒的扩增量。作用96 h时，无论是中和实验还是增殖抑制实验，石油醚萃取物的作用都是最显著的，病毒扩增率较阳性对照分别减少了92.4%和95.6%。结论 蒲公英乙酸乙酯萃取物和石油醚萃取物在体外有明显的抗甲型H1N1流感病毒的作用。 |
| 关键词: 蒲公英 甲型流感病毒 狗肾传代细胞 抗病毒作用 萃取物 |
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| 基金项目:国家基金(81460593，81171946)；云南省科技计划项目(2014FA031，2011CA016，2012FB188)；中国医学科学院医学生物学研究所科技计划重点项目(2014IMB05ZD) |
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| Anti-influenza A (H1N1) Virus Effect of Organic Solvent Extracts from Taraxaci Herba |
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WANG Xiaodan1, XIA Xiaoling2, ZHAO Yujiao1, WANG Xinhua3, ZHANG Rongping2, SUN Qiangming1
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1.Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Disease, Kunming 650118, China;2.Kunming Medical University, Kunming 650500, China;3.The First Affiliated Hospital of Guangzhou Medical University, National Key Laboratory of Respiratory Disease, Guangzhou 510230, China
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| Abstract: |
| OBJECTIVE To investigate the anti-influenza A (H1N1) virus effect of organic?solvent extracts from Taraxaci Herba by neutralization test and proliferation inhibition test in vitro. METHODS After Taraxaci Herba was extracted by alcoholate, the alcoholate extracts of Taraxaci Herba were extracted by ethyl acetate, n-butyl alcohol, petroleum ether and water in turn. Cytopathogenic effect (CPE) of MDCK cell infection by influenza A (H1N1) virus was observed with the interference of Taraxaci Herba extract based on cell culture technique. The anti-influenza A (H1N1) virus effect of neutralization test and proliferation inhibition test of Taraxaci Herba extracts were observed by the hemagglutination titre test and Real time RT-PCR. RESULTS Anti-influenza A (H1N1) virus neutralization and proliferation inhibition effect of four Taraxaci Herba extracts were observed after 96 h of treatment. The results of neutralization test showed that hemagglutination titer of ethyl acetate extracts were 2-4 times lower than the control group. While the results of neutralization and proliferation inhibition test showed that hemagglutination titer of petroleum ether extract was 256 and 128 times lower than the control group, respectively. According to the results of Real time RT-PCR test, the inhibition ratio of ethyl acetate extract and petroleum ether extract were lower than the control group. After 96 h of treatment by petroleum ether extract, amplification inhibition rate of neutralization and proliferation inhibition test were 92.4% and 95.6% lower comparing to the control, respectively. CONCLUSION ethyl acetate and petroleum ether extract of Taraxaci Herba have anti-influenza A (H1N1) virus effects in vitro. |
| Key words: Taraxaci Herba influenza A virus MDCK cells antiviral effect extract |
