| 引用本文: | 王庆振,李莉.毛细管微萃取结合微顺序注射在线检测全血中异丙酚含量[J].中国现代应用药学,2016,33(1):16-19. |
| WANG Qingzhen,LI Li.Online Determination of Propofol in Whole Blood by Combining Capillary Micro-extraction with Micro-sequential Injection[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(1):16-19. |
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| 摘要: |
| 目的 建立一种快速测定全血中异丙酚含量的原位、在线分析的新方法。方法 利用毛细管微萃取器联合紫外光纤传感仪及微顺序注射-阀上实验室系统在线测定全血中异丙酚含量。结果 该方法的加样回收率为95.8%~103.6%；低、中、高 3 个浓度(100，150，200 μg·mL-1)的日内精密度RSD分别为7.2%，8.2%和2.6%，日间精密度RSD分别为10.0%，6.1%和7.7%。与传统液液萃取方法相比，该方法异丙酚平均萃取率提高3.5%，萃取时间缩短5~7 min。结论 该方法样品前处理无需离心、除蛋白等操作, 能快速测定全血中异丙酚的含量, 为全血中异丙酚含量原位、在线检测提供了良好的手段。 |
| 关键词: 异丙酚 光纤化学传感 毛细管微萃取器 微顺序注射-阀上实验室系统 |
| DOI： |
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| 基金项目:国家自然科学基金(81260485) |
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| Online Determination of Propofol in Whole Blood by Combining Capillary Micro-extraction with Micro-sequential Injection |
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WANG Qingzhen, LI Li
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Xinjiang Medical University, Urumqi 830011, China
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| Abstract: |
| OBJECTIVE To determine the propofol’s content in whole blood online and in situ by combined capillary micro-extraction rapid extraction with optical-fiber. METHODS Capillary micro-extractor, optical-fiber sensing devices and microSIA lab-on-valve were used to determine the propofol in whole blood. RESULTS The new methods of recovery ranged in 95.8%-103.6%. The intra-day RSDs were 7.2%, 8.2% and 2.6%, and inter-day RSDs were 10.0%, 6.1% and 7.7%, respectively at 3 concentrations(100, 150, 200 μg·mL-1). The?average?extraction?rate was higher than tradition liquid-liquid extraction about 3.5%, and the extraction time was still shorter about 5-7 min. CONCLUSION This new method could test propofol in whole blood online and in situ without using centrifuge and removing protein, and provide an excellent opition to determine?the?propofol?in?whole?blood. |
| Key words: propofol optical-fiber chemical sensor capillary micro-extractor microSIA lab-on-valve |
