| 引用本文: | 王有琼,马李一,张重权,张燕平,段琼芬.微波辅助提取印楝叶多酚及其体外抗氧化活性研究[J].中国现代应用药学,2015,32(11):1319-1324. |
| WANG Youqiong,MA Liyi,ZHANG Zhongquan,ZHANG Yanping,DUAN Qiongfen.Microwave Extraction Technique of Polyphenols and in Vitro Antioxidation Activity from the Leaves of Azadirachta Indica A.Juss.[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(11):1319-1324. |
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| 摘要: |
| 目的 筛选微波辅助提取印楝叶总多酚的工艺条件，并测定印楝叶总多酚的体外抗氧化活性。方法 通过单因素和响应曲面法设计实验，考察萃取温度、萃取时间、乙醇体积分数3个响应变量以及变量之间交互作用对印楝叶多酚提取效果的影响，对提取工艺进行优化。以清除2,2-联苯基-1-苦基肼基(DPPH·)自由基、羟自由基(·OH)及还原力测定实验评价印楝叶多酚的抗氧化能力。结果 微波辅助印楝叶多酚提取的最佳工艺条件为萃取温度62 ℃，萃取时间40 min，乙醇体积分数48%；响应变量影响顺序为萃取温度>乙醇体积分数>萃取时间；验证实验得到印楝叶中多酚提取得率平均为2.96%，与理论值相对误差为1.33%。印楝叶多酚清除DPPH·自由基的半数抑制质量浓度(IC50)约为2.6 mg·L-1，·OH自由基的IC50约为15 mg·L-1，在较低浓度时印楝叶多酚具有较强还原能力。结论 印楝叶多酚在低浓度时表现出较维生素C更强的抗氧化活性。 |
| 关键词: 印楝 多酚 微波萃取 抗氧化 |
| DOI： |
| 分类号:TQ91；R284.2 |
| 基金项目:“十二五”农村领域国家科技计划(2012BAD21B04) |
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| Microwave Extraction Technique of Polyphenols and in Vitro Antioxidation Activity from the Leaves of Azadirachta Indica A.Juss. |
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WANG Youqiong, MA Liyi, ZHANG Zhongquan, ZHANG Yanping, DUAN Qiongfen
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Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, China
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| Abstract: |
| OBJECTIVE To optimize the microwave extraction technique of polyphenol from Azadirachta indica A. Juss. leaves by response surface methodology and to evaluate the in vitro antioxidant activity of polyphenol in leaves of Azadirachta indica A.Juss.. METHODS The experiment was designed by single factor experiment and response surface method. The influence and the interaction of three response variables such as extract temperature, extraction time, ethanol concentration were investigated. The extraction technology was optimized. The antioxidant activities of polyphenol extracted from Azadirachta indica A. Juss. leaves were evaluated through clearing the DPPH·, ·OH free radical and reducing power. RESULTS The optimum microwave extraction conditions of polyphenol in the Azadirachta indica A. Juss. leaves were extraction temperature 62 ℃, extraction time 40 min, ethanol concentration 48%. The influence order of response variables was extraction temperature, ethanol concentration, extraction time. The average extract ratio of Azadirachta indica A. Juss. leaves polyphenol was 2.96% and the relative error was 1.33%. The IC50 values of Azadirachta indica A. Juss. leaves phenolics scavenging activity on DPPH was about 2.6 mg·L-1. The IC50 values of Azadirachta indica A. Juss. leaves phenolics scavenging activity on ·OH was about 15 mg·L-1. The reduction ability of Azadirachta indica A. Juss. leaves polyphenols was strong at lower concentration. CONCLUSION The antioxidant activities of polyphenol extracted from Azadirachta indica A. Juss. leaves were stronger than that of Vc at low concentration. |
| Key words: azadirachta indica polyphenol microwave extraction antioxidant |
