| 引用本文: | 郑明,金情政,陈闯,江明,徐付珍,宋剑锋.HPLC同时测定五福化毒丸中靛蓝、靛玉红和甘草酸的含量[J].中国现代应用药学,2016,33(1):76-78. |
| ZHENG Ming,JIN Qingzheng,CHEN Chuang,JIANG Ming,XU Fuzhen,SONG Jianfeng.Simultaneous Determination of Indigo, Indirubin and Glycyrrhizic Acid in Wufu Huadu Pill by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(1):76-78. |
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| 摘要: |
| 目的 建立同时测定五福化毒丸中靛蓝、靛玉红和甘草酸含量的高效液相色谱法。方法 采用Eclipse XDB-C18(250 mm×4.6 mm，5 μm)色谱柱，流动相为甲醇-0.2 mol·L-1乙酸铵-冰醋酸(65∶35∶1)，流速为1.0 mL·min-1，检测波长为288，250 nm，柱温：25 ℃。结果 靛蓝、靛玉红和甘草酸依次在2.501~25.01，0.633~6.330，5.10~51.02 μg·mL-1内与峰面积呈良好线性关系，相关系数r分别为1.000 0，0.999 5，1.000 0，加样回收率(n=9)分别为98.3%，98.4%和98.2%。结论 本方法简便，准确，重复性好，可用于该制剂的质量控制。 |
| 关键词: 五福化毒丸 靛蓝 靛玉红 甘草酸 高效液相色谱法 |
| DOI： |
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| 基金项目:浙江省衢州市重点实验室项目(衢市科发高〔2015〕8号) |
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| Simultaneous Determination of Indigo, Indirubin and Glycyrrhizic Acid in Wufu Huadu Pill by HPLC |
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ZHENG Ming1, JIN Qingzheng2, CHEN Chuang2, JIANG Ming2, XU Fuzhen2, SONG Jianfeng2
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1.Quzhou Institute for Food and Drug Control, Quzhou 324000, China;2.Zhejiang Quhua Hospital, Quzhou 324004, China
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| Abstract: |
| OBJECTIVE To develop an HPLC method for simultaneous determination of indigo, indirubin and glycyrrhizic acid in Wufu Huadu pill. METHODS An Eclipse XDB-C18 column(250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was methanol-0.2 mol·L-1 ammonium acetate-glacial acetic acid(65∶35∶1) at the flow rate of 1.0 mL·min-1. The UV-detection wavelength was at 250, 288 nm with the column temperature of 25 ℃. RESULTS Calibration curves were found to be linear in the ranges of 2.501-25.01 μg·mL-1 for indigo, 0.633-6.330 μg·mL-1 for indirubin and 5.10-51.02 μg·mL-1 for glycyrrhizic acid. The correlation coefficients were 1.000 0, 0.999 5, 1.000 0. The average recoveries(n=9) of indigo, indirubin and glycyrrhizic acid were 98.3%, 98.4% and 98.2%. CONCLUSION The method is simple, accurate and reproducible. It could be reliable for the quality control of Wufu Huadu Pill. |
| Key words: Wufu Huadu pill indigo indirubin glycyrrhizic acid HPLC |
