| 引用本文: | 黄丽萍,黄丽英,李喜载,俞晓玲.银线莲药材HPLC指纹图谱研究[J].中国现代应用药学,2015,32(10):1170-1174. |
| HUANG Liping,HUANG Liying,LI Xizai,YU Xiaolin.Fingerprint Chromatography of Goodyera matsumurana by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(10):1170-1174. |
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| 摘要: |
| 目的 建立银线莲的HPLC指纹图谱分析方法，为规范银线莲药材的质量标准提供基础研究资料。方法 采用Agilent TC-C18色谱柱(4.6 mm×250 mm，5 μm)，流动相为甲醇-0.02 mol·L-1乙酸铵溶液，梯度洗脱，流速：1.0 mL·min-1，检测波长：260 nm，柱温：30 ℃。结果 10批银线莲指纹图谱的相似度均>0.90，确定了20个共有峰，指认了4个特征峰，建立了该药材的高效液相色谱指纹图谱。结论 该指纹图谱方法准确可靠，重复性好，能很好地区分开金线莲、银线莲，可作为银线莲药材内在质量评价的依据。 |
| 关键词: 银线莲 高效液相色谱法 指纹图谱 |
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| 基金项目:国家卫生和计划生育委员会科研基金课题(wkj-FJ-33)；福建省自然基金项目(2012J01368)；福建医科大学教授学术发展基金(JS09005) |
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| Fingerprint Chromatography of Goodyera matsumurana by HPLC |
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HUANG Liping1, HUANG Liying2, LI Xizai3, YU Xiaolin2
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1.The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China;2.College of Pharmacy, Fujian Medical University, Fuzhou 350004, China;3.College of Sitiology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
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| Abstract: |
| OBJECTIVE To establish fingerprint chromatography of Goodyera matsumurana by HPLC and provide a basic research data for the quality standard of Goodyera matsumurana. Methods Agilent TC-C18 column(4.6 mm×250 mm, 5 μm) was adopted. The mobile phase was methanol-0.02 mol·L-1 ammonium acetate with gradient elution at the flow rate of 1.0 mL·min-1. The detection wave length was 260 nm. The column temperature was 30 ℃. RESULTS The similarity of 10 batches of Goodyera matsumurana was >0.90. Twenty common peaks were indicated. Four peaks were identified. The fingerprint chromatography of Goodyera matsumurana was established. Conclusion This method is s accurate and repeatable for the quality control of Goodyera matsumurana. It can well distinguish Goodyera matsumurana from Anoectochilus formosanus. |
| Key words: Goodyera matsumurana HPLC fingerprint chromatography |
