| 引用本文: | 黄涵,曾令杰,莫运才,冯鸿耀,廖素溪,郭玉梅,李振华.响应面法优化超声波提取昆仑雪菊总黄酮的工艺研究[J].中国现代应用药学,2015,32(8):947-951. |
| HUANG Han,ZENG Lingjie,MO Yuncai,FENG Hongyao,LIAO Suxi,GUO Yumei,LI Zhenhua.Optimization of Ultrasonic Extraction of Total Flavonoids from Coreopsis Tinctoria Nutt. Using Response Surface Methodology[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(8):947-951. |
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| 摘要: |
| 目的 优化昆仑雪菊的超声提取工艺。方法 以总黄酮提取量为指标，在单因素试验基础上，通过Box-Behnken响应面法考察乙醇体积分数、料液比、超声时间和温度对总黄酮提取效果的影响。结果 最佳超声提取工艺为乙醇体积分数65%，料液比40 g·mL-1，超声时间34 min，超声温度62 ℃；总黄酮提取量为207.32 mg·g-1(RSD=1.45%)，与预测值(208.81 mg·g-1)的偏差较小。结论 优选的提取工艺可行，为昆仑雪菊的开发应用提供实验依据。 |
| 关键词: 昆仑雪菊 总黄酮 超声波提取 单因素试验 Box-Behnken响应面 |
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| Optimization of Ultrasonic Extraction of Total Flavonoids from Coreopsis Tinctoria Nutt. Using Response Surface Methodology |
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HUANG Han, ZENG Lingjie, MO Yuncai, FENG Hongyao, LIAO Suxi, GUO Yumei, LI Zhenhua
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Guangdong Pharmacy University, Guangzhou 510006, China
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| Abstract: |
| OBJECTIVE To optimize ultrasound extraction technology of total flavonoids from Coreopsis tinctoria Nutt.. METHODS With the content of total flavonoids as index, effects of ethanol concentration, solid-liquid ratio, ultrasonic time and temperature on extraction technology were investigated by single factor tests and Box-Behnken response surface methodology. RESULTS Optimum ultrasound extraction technology was as following: ultrasonic extracted 34 min with 40 times of 65% ethanol to the herb at 62 ℃. Under these conditions, the average extraction rate of flavonoids was 207.32 mg·g-1(RSD=1.45%), which was very close to the predicted value of 208.81 mg·g-1. CONCLUSION This optimized technology is stable and feasible to provide a basis for development and application of total flavonoids in Coreopsis tinctoria Nutt.. |
| Key words: Coreopsis tinctoria Nutt. total flavonoids ultrasonic extraction single fator test Box-Behnken response surface methodology |
