| 引用本文: | 喻录容,何先元,黄英如.HPLC同时测定冠心康颗粒中芍药苷、丹酚酸B和羟基红花黄色素A的含量[J].中国现代应用药学,2015,32(5):561-564. |
| YU Lurong,HE Xianyuan,HUANG Yingru.Simultaneous Determination of Paeoniflorin, Salvianolic Acid B and Hydroxysafflor Yellow A in Guanxinkang Granules by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(5):561-564. |
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| 摘要: |
| 目的 建立同时测定冠心康颗粒中芍药苷、丹酚酸B和羟基红花黄色素A含量的方法。方法 采用Welchrom C18色谱柱(250 mm×4.6 mm，5 μm)，以乙腈(A)-0.1%磷酸(B)为流动相，梯度洗脱；流速：1.0 mL·min-1；检测波长：230 nm。结果 芍药苷在7.02~52.64 μg·mL-1内呈良好的线性关系(r=0.999 7)，平均回收率为99.3%，RSD为1.2%；丹酚酸B在23.19~173.90 μg·mL-1内呈良好的线性关系(r=0.999 7)，平均回收率为98.2%，RSD为1.1%；羟基红花黄色素A在4.47~33.50 μg·mL-1内呈良好的线性关系(r=0.999 3)，平均回收率为98.0%，RSD为1.0%。结论 本法简便、灵敏、准确、重复性好，可用于冠心康颗粒的质量控制。 |
| 关键词: 冠心康颗粒 芍药苷 丹酚酸B 羟基红花黄色素A 高效液相色谱法 |
| DOI： |
| 分类号:R284.1 |
| 基金项目:国家自然科学基金项目(81373668)；重庆市卫生局科技项目(2012-2-144) |
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| Simultaneous Determination of Paeoniflorin, Salvianolic Acid B and Hydroxysafflor Yellow A in Guanxinkang Granules by HPLC |
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YU Lurong, HE Xianyuan, HUANG Yingru
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Institute of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 401331, China
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| Abstract: |
| OBJECTIVE To establish a simultaneous determination method for paeoniflorin, salvianolic acid B and hydroxysafflor yellow A in Guanxinkang granules. METHODS The determination was performed on a Welchrom C18 column(250 mm×4.6 mm, 5 μm) with acetonitrile(A)-0.1% phosphoric acid(B) as the mobile phase in gradient elution mode at the flow rate of 1.0 mL·min-1. The detection wavelength was 230 nm. RESULTS Paeoniflor presented a good linearity in the concentration range of 7.02-52.64 μg·mL-1. The average rate of recovery was 99.3 % with RSD of 1.2%. Salvianolic acid B presented a good linearity in the concentration range of 23.19-173.90 μg·mL-1. The average rate of recovery was 98.2% with RSD of 1.1%. Hydroxysafflor yellow A presented a good linearity in the concentration range of 4.47-33.50 μg·mL-1. The average rate of recovery was 98.0% with RSD of 1.0%. CONCLUSION This method was simple, accurate and reproducible, it can be used as the basis for quality control of Guanxinkang granules. |
| Key words: Guanxinkang granules paeoniflorin salvianolic acid B hydroxysafflor yellow A HPLC |
