| 引用本文: | 栾连军,雷后亮,周芸,刘忠达,袁宙新,吴永江.莲房原花青素提取纯化工艺研究与抗氧化活性评价[J].中国现代应用药学,2015,32(9):1069-1073. |
| LUAN Lianjun,LEI Houliang,ZHOU Yun,LIU Zhongda,YUAN Zhouxin,WU Yongjiang.Extraction and Purification of Proanthocyanidins from Nelumbinis Receptaculum and Evaluation of Its Antioxidant Activity[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(9):1069-1073. |
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| 摘要: |
| 目的 优化莲房原花青素提取纯化工艺并评价抗氧化活性。方法 以正交设计L9(34)优化莲房原花青素提取工艺参数，用大孔吸附树脂进行纯化，而后通过1,1-二苯基-2-苦肼基(DPPH)和羟基自由基(·OH)清除率评价其体外抗氧化活性。结果 在最佳提取工艺条件下(8倍量50%乙醇回流提取2次，每次3 h)，提取率达91.3%，进一步通过大孔树脂AB-8纯化，最终提取物中原花青素纯度为80.7%。莲房原花青素抗氧化活性与葡萄籽原花青素无显著性差异，均明显优于维生素C。结论 该提取纯化方法简单、高效，可用于莲房原花青素提取物的制备。莲房原花青素具有较强的抗氧化活性，值得进一步开发利用。 |
| 关键词: 莲房 原花青素 提取 纯化 抗氧化活性 |
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| 基金项目:丽水市科技计划项目(20110411) |
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| Extraction and Purification of Proanthocyanidins from Nelumbinis Receptaculum and Evaluation of Its Antioxidant Activity |
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LUAN Lianjun1, LEI Houliang1, ZHOU Yun1, LIU Zhongda2, YUAN Zhouxin2, WU Yongjiang1
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1.College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;2.Lishui Hospital of Traditional Chinese Medicine, Lishui 323000, China
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| Abstract: |
| OBJECTIVE To optimize the extraction and purification conditions of proanthocyanidins from Nelumbinis Receptaculum and to evaluate its antioxidant activity. METHODS An orthogonal experiment L9(34) was applied to obtain the best extraction conditions, and macroporous adsorption resin was used to purify the crude extract. Furthermore, the antioxidant activities of proanthocyanidins from Nelumbinis Receptaculum and grape seed were evaluated with two different in vitro assays methods: scavenging effect on 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals. RESULTS The highest extraction yield of 91.3% was obtained when extraction times, ratio of solvents to raw material and concentration of ethanol were 3 h, 8 and 50%, respectively. And the crude extract was purified with macroporous adsorption resin AB-8 to gain the final extract with proanthocyanidins purity of 80.7%. The antioxidant activity of proanthocyanidins extract from Nelumbinis Receptaculum was similar to that from grape seed, which was much better than vitamin C. CONCLUSION An extraction and purification process with procedural simplicity, high efficiency is established for the preparation of Nelumbinis Receptaculum proanthocyanidins extract which can be futher developed because of its strong antioxidant activity. |
| Key words: Nelumbinis Receptaculum proanthocyanidins extraction purification antioxidant activity |
