| 引用本文: | 韦永爱,施宏刚,高鹍,吴海燕,李轩,董伟.HPLC测定甲磺酸加雷沙星异构体[J].中国现代应用药学,2015,32(7):840-843. |
| WEI Yongai,SHI Honggang,GAO Kun,WU Haiyan,LI Xuan,DONG Wei.Determination of Enantiomers in Garenoxacin Mesylate by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(7):840-843. |
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| 摘要: |
| 目的 建立甲磺酸加雷沙星异构体的HPLC检测方法。方法 Chiralpak AD-H色谱柱(250 mm×4.6 mm，5 μm)，柱温：35 ℃，检测波长：278 nm，流速：1.0 mL·min-1，流动相：正己烷-异丙醇-甲醇-甲磺酸(70∶29∶1∶0.1)。结果 甲雷沙星及其异构体在0.005~0.151 μg·mL-1内具有良好的线性(r>0.999)，定量限浓度为5 ng·mL-1(相当于0.03%)，最低检测限为2.5 ng·mL-1(相当于0.015%)。结论 该方法简便、准确，具有良好的重现性和专属性。 |
| 关键词: 甲磺酸加雷沙星 异构体 高效液相色谱法 |
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| Determination of Enantiomers in Garenoxacin Mesylate by HPLC |
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WEI Yongai, SHI Honggang, GAO Kun, WU Haiyan, LI Xuan, DONG Wei
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Tianjin Hankang Medicinal & Biological Technology Co.,Ltd., Tianjin 300409, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for determining of enantiomers in garenoxacin mesylate. METHODS The column was Chiralpak AD-H (250 mm×4.6 mm, 5 μm) was used. Column temperature was 35 ℃, detection wavelength was 278 nm, flow rate was 1.0 mL·min-1, and the mobile phase consisted of n-hexane-isopropanol-methanol-methanesulfonic acid(70∶29∶1∶0.1). RESULTS The calibration curves of garenoxacin and enantiomers were linear over the range of 0.005-0.151 μg·mL-1(r>0.999). The limit of quantification was 5 ng·mL-1(equivalent to 0.03%). The minimum detection limit was 2.5 ng·mL-1(equivalent to 0.015%). CONCLUTION The method is sample, accurate, reproducible and specific for determination of ethyl methanesulfonate in carenoxacin mesylate. |
| Key words: garenoxacin mesylate enantiomers HPLC |
