右美托咪定预处理对局灶性脑缺血/再灌注星形胶质细胞胶质纤维酸性蛋白表达的影响

尚宇; 顾佩菲<sup>*</sup>; 袁士涛; 李悦; 高光洁

引用本文:	尚宇,顾佩菲,袁士涛,李悦,高光洁.右美托咪定预处理对局灶性脑缺血/再灌注星形胶质细胞胶质纤维酸性蛋白表达的影响[J].中国现代应用药学,2015,32(7):799-803.
	SHANG Yu,GU Peifei,YUAN Shitao,LI Yue,GAO Guangjie.Effects of Dexmedetomidine on Astrocytes Glial Fibrillary Acidic Protein Expression in Focal Cerebral Ischemia/Reperfusion Rats[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(7):799-803.

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右美托咪定预处理对局灶性脑缺血/再灌注星形胶质细胞胶质纤维酸性蛋白表达的影响
尚宇¹, 顾佩菲², 袁士涛¹, 李悦³, 高光洁¹
1.解放军第463医院，沈阳 110042;2.东北制药集团沈阳第一制药有限公司，沈阳 110016;3.哈尔滨医科大学附属一院，哈尔滨 150001

摘要:

目的探讨预注右美托咪定对局灶性脑缺血/再灌注后星形胶质细胞胶质纤维酸性蛋白(glial fibrillary acidic protein，GFAP)表达的影响。方法大脑中动脉插线法制作大鼠局灶性脑缺血/再灌注模型。观察脑缺血2 h再灌注24 h后神经功能损害改变并评分，并采用免疫组化和蛋白免疫印迹方法观察大鼠脑缺血后星形胶质细胞GFAP蛋白表达情况。结果缺血/再灌注后可诱导大鼠脑组织星形胶质细胞GFAP表达明显增强，右美托咪定可明显改善大鼠神经功能损害，减少缺血区GFAP阳性星型胶质细胞，降低GFAP表达水平。结论早期应用右美托咪定可抑制脑缺血后星形胶质细胞GFAP的过度表达，可发挥抗脑缺血损伤，保护神经元作用。

关键词: 右美托咪定缺血/再灌注胶质纤维酸性蛋白星形胶质细胞

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Effects of Dexmedetomidine on Astrocytes Glial Fibrillary Acidic Protein Expression in Focal Cerebral Ischemia/Reperfusion Rats

SHANG Yu¹, GU Peifei², YUAN Shitao¹, LI Yue³, GAO Guangjie¹

1.463 Hospital of PLA, Shenyang 110042, China;2.Northeast Pharmaceutical Group Shenyang First Pharmaceutical Company, Shenyang 110016, China;3.First-affiliated Hospital of Harbin Medical University, Harbin 150001, China

Abstract:

OBJECTIVE To investigate astrocyte glial fibrillary acidic protein(GFAP) expression on focal cerebral ischemia/reperfusion rats with pre-infusion of dexmedetomidine. METHODS Middle cerebral artery suture method was used to establish focal cerebral ischemia/reperfusion model. Observation nerve function change and score at cerebral ischemia 2 h and reperfusion 24 h, GFAP protein expression in the dentate gyrus of rats were examined by immunohistochemistry method. RESULTS Ischemia/reperfusion could enhance GFAP expression in dentate gyrus, dexmedetomidine could improve the neurological function injury and reverse GFAP expression in rats significantly(P<0.05). CONCLUSION Pre-application of dexmedetomidine can inhibit the astrocyte GFAP overexpression and play a protective effect against cerebral ischemic injury.

Key words: dexmedetomidine ischemia/reperfusion glial fibrillary acidic protein (GFAP) astrocyte