柳茶提取物对异烟肼引起的肝损伤小鼠CYP2E1酶活性及mRNA表达的影响

武燕; 邓婉蓉; 何华; 刘健

引用本文:	武燕,邓婉蓉,何华,刘健.柳茶提取物对异烟肼引起的肝损伤小鼠CYP2E1酶活性及mRNA表达的影响[J].中国现代应用药学,2015,32(7):795-799.
	WU Yan,DENG Wanrong,HE Hua,LIU Jian.Effect of Extracts of Sibraea Angustata (Rchd.) Hand-Mazz. on the Enzyme Activity and mRNA Expression of CYP2E1 in Mice with Isoniazid-induced Liver Injury[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(7):795-799.

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柳茶提取物对异烟肼引起的肝损伤小鼠CYP2E1酶活性及mRNA表达的影响
武燕¹, 邓婉蓉¹, 何华¹, 刘健²
1.甘肃中医学院，兰州 730030;2.兰州大学第一附属医院，兰州 730030

摘要:

目的研究柳茶提取物对异烟肼引起小鼠肝损伤的CYP2E1基因表达的影响。方法 60只小鼠随机分为正常对照组、模型对照组、阳性对照组(水飞蓟宾胶囊)和柳茶提取物低、中、高剂量组(1.0，1.5，2.0 g·kg^-1)，分别灌胃给予相应的药物，连续10 d，检测各组血清(ALT、AST)及肝脏相关生化指标以及肝脏组织(MDA、SOD、GSH-PX）、病理学变化、CYP2E1酶活性及mRNA表达。结果与模型组比较，柳茶提取物组血清及肝脏相关生化指标有显著改善(P<0.05或0.001)，病理学研究进一步证明了其保护作用，CYP2E1的酶活性及mRNA表达均明显降低(P<0.05)。结论柳茶提取物可能通过抑制CYP2E1的活性和表达来减少自由基的产生，从而达到减轻异烟肼引起的小鼠氧化性肝损伤作用。

关键词: 柳茶提取物肝损伤异烟肼 CYP2E1

DOI：

分类号:

基金项目:甘肃中医学院中青年科研基金项目(ZQ2014-6)

Effect of Extracts of Sibraea Angustata (Rchd.) Hand-Mazz. on the Enzyme Activity and mRNA Expression of CYP2E1 in Mice with Isoniazid-induced Liver Injury

WU Yan¹, DENG Wanrong¹, HE Hua¹, LIU Jian²

1.Gansu College of Traditional Chinese Medicine, Lanzhou 730030, China;2.The First Hospital of Lanzhou University, Lanzhou 730030, China

Abstract:

OBJECTIVE To explore the effect of the extracts of Sibraea angustata (ESA) on gene expression of CYP2E1 in mice with liver injury induced by isoniazid(INH). METHODS Sixty mice were randomly divided into normal control group, model control group, positive control group (Silybin capsules) and three doses(1.0, 1.5, 2.0 g·kg^-1) groups of ESA. After intragastric administrated with corresponding drugs for 10 days, the biochemical indicators in surum(AST, ALT) and liver (MDA, SOD, GSH-PX), histological patterns, the activity and mRNA epression of CYP2E1 in mice were measured and compared. RESULTS Compared with model control group, the activity of related biochemical indexs ameliorated significantiy(P<0.05 or 0.001), which were confirmed by histopathological analysis. The enzyme activity and mRNA expression of CYP2E1 decreased significantly in ESA-treated groups (P<0.05). CONCLUSION ESA may exerts its hepatoprotective activity against INH-induced liver injury by inhibiting the activity and expression of CYP2E1 andproducyion free radical.

Key words: extracts of Sibraea angustata (Rchd.) Hand-Mazz. liver injury isoniazid CYP2E1