| 引用本文: | 范蕾,吴查青,王伟影,陈卫平,毛菊华,陈张金,李水福.市售枳壳饮片的质量调查与指纹图谱研究[J].中国现代应用药学,2015,32(4):432-436. |
| FAN Lei,WU Chaqing,WANG Weiying,CHEN Weiping,MAO Juhua,CHEN Zhangjin,LI Shuifu.Study on the Quality Investigation and Fingerprint of Aurantii Fructus[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(4):432-436. |
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| 摘要: |
| 目的 建立HPLC指纹图谱，调查16批市售枳壳饮片的质量。方法 色谱柱为Agilent-SB C18(250 mm×4.6 mm，5 μm)，流动相为乙腈-水(20︰80)(用磷酸调节pH值至3)，流速为1.0 mL?min-1，柱温30 ℃，检测波长为283 nm。利用相似度评价系统进行指纹图谱相似度计算。结果 16批市售枳壳饮片均达到药典标准，不同炮制方法所得枳壳含量略有差异。枳壳指纹图谱相似度>0.98。结论 市售枳壳饮片质量较好。指纹图谱方法稳定、重复性好，可用于枳壳的质量控制。 |
| 关键词: 枳壳 质量 指纹图谱 |
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| 基金项目:浙江省公共科技条件平台首期建设项目(2012E60008)；丽水市重点科技创新团队建设项目(2012CXTD11) |
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| Study on the Quality Investigation and Fingerprint of Aurantii Fructus |
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FAN Lei, WU Chaqing, WANG Weiying, CHEN Weiping, MAO Juhua, CHEN Zhangjin, LI Shuifu
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Lishui Institute for Food and Drug Control, Lishui 323000, China
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| Abstract: |
| OBJECTIVE To investigate the quality of the 16 batches of commercial Aurantii Fructus by establishment of HPLC fingerprinting. METHODS HPLC was applied with the chromatographic condition as follows: the chromatographic column was Agilent Zorbax SB-C18 (250 mm×4.6 mm, 5 μm) at 30 ℃; mobile phase was acetonitrile-water(20∶80)(adjusted to pH 3 with phosphoric acid); flow rate was 1.0 mL?min-1, the detection wavelength was 283 nm. Used the similarity evaluation system for fingerprint similarity calculation. RESULTS Sixteen batches of commercial Aurantii Fructus were reaching pharmacopoeia standards, there were a little differences between two processing methods. Fingerprint similarity was above 0.98. CONCLUSION The method of fingerprint is stable, repeatable and it is suitable for the quality control of Aurantii Fructus. |
| Key words: Aurantii Fructus quality fingerprint |
