| 引用本文: | 郑娟,茅纯.HPLC波长转换法同时测定小儿咳喘灵颗粒中(R,S)-告依春、绿原酸和甘草苷的含量[J].中国现代应用药学,2015,32(6):727-731. |
| ZHENG Juan,MAO Chun.Simultaneous Determination of Epigoitrin, Chlorogenic Acid and Liquiritin in Child Kechuanling Granules by Wavelength Conversion HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(6):727-731. |
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| 摘要: |
| 目的 建立高效液相色谱波长转换法同时测定小儿咳喘灵颗粒中(R,S)-告依春、绿原酸和甘草苷含量的方法。方法 样品采用70%甲醇溶液超声提取，采用Inertsil ODS-SP C18色谱柱(4.6 mm×250 mm，5 μm)分离；流动相：0.1%磷酸-乙腈，梯度洗脱；流速为1.0 mL·min-1；检测波长：0~13 min为245 nm，13~50 min为217 nm。结果 (R,S)-告依春、绿原酸和甘草苷分别在0.700~14.000 μg·mL-1、4.780~95.600 μg·mL-1、1.050~21.008 μg·mL-1内具有良好的线性关系；加样回收率分别为98.8%，102.8%和97.7%；RSD分别为1.2%，1.9%和1.4%(n=9)。结论 该方法简单易行，准确性和重复性好，可用于该制剂的质量控制。 |
| 关键词: 高效液相色谱 小儿咳喘灵颗粒 (R,S)-告依春 绿原酸 甘草苷 |
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| Simultaneous Determination of Epigoitrin, Chlorogenic Acid and Liquiritin in Child Kechuanling Granules by Wavelength Conversion HPLC |
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ZHENG Juan, MAO Chun
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Hangzhou Institute for Drug Control, Hangzhou 310015, China
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| Abstract: |
| OBJECTIVE To determine the content of epigoitrin, chlorogenic acid and liquiritin in child kechuanling granules by wavelength conversion HPLC. METHODS The sample was extracted by 70% methanol and separated on a Inertsil ODS-SP C18(4.6 mm×250 mm, 5 μm). The mobile phase were 0.1% phosphoric acid and acetonitrile, gradient elution. The flow rate was 1.0 mL·min-1 while the ultraviolet detector was operated at 245 nm(0-13 min) and 217 nm(13-52 min). RESULTS The linear range of epigoitrin, chlorogenic acid and liquiritin was respectively in 0.700-14.000 μg·mL-1, 4.780-95.600 μg·mL-1, 1.050-21.008 μg·mL-1 while the average recovery was 98.8%, 102.8%, 97.7% with the RSD 1.2%, 1.9%, 1.4%(n=9). CONCLUSION This method is simple, rapid and with good repeatability, so it could be used in quality control of child kechuanling granules. |
| Key words: HPLC Child Kechuanling granules epigoitrin chlorogenic acid liquiritin |
