| 引用本文: | 冷晓红,郭鸿雁,陈海燕.苦豆子提取过程中氧化苦参碱与苦参碱、氧化槐果碱与槐果碱的相互转化[J].中国现代应用药学,2015,32(6):688-691. |
| LENG Xiaohong,GUO Hongyan,CHEN Haiyan.Study on Transformation Between Matrine and Oxymatrine, Oxysophocarpine and Sophocarpine in the Extraction Process of Sophora Alopecuroides[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(6):688-691. |
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| 摘要: |
| 目的 研究苦豆子药用植物提取过程中氧化苦参碱与苦参碱、氧化槐果碱与槐果碱的相互转化。方法 采用不同提取溶媒(水及55%乙醇)、不同pH及不同提取时间3个因素，HPLC测定苦豆籽、苦豆草植物中氧化苦参碱与苦参碱，氧化槐果碱与槐果碱的含量。结果 随着提取时间的增加，氧化型生物碱转化为还原型生物碱的量随之增大；提取溶剂对苦豆子中生物碱转化的影响无明显差异；随着提取时间的增加，氧化苦参碱与苦参碱总量呈缓慢下降的趋势，氧化槐果碱与槐果碱总量也呈缓慢下降的趋势。结论 氧化型生物碱与还原型生物碱转化趋势可为苦豆子生物碱质量控制提供依据，也可为工业化提取苦豆子生产工艺提供依据。 |
| 关键词: 苦参碱 氧化苦参碱 槐果碱 氧化槐果碱 相互转化 |
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| 基金项目:科技部国家科技支撑项目(2011BAI05B00) |
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| Study on Transformation Between Matrine and Oxymatrine, Oxysophocarpine and Sophocarpine in the Extraction Process of Sophora Alopecuroides |
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LENG Xiaohong, GUO Hongyan, CHEN Haiyan
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Ningxia Vocational and Technical College, Yinchuan 750021, China
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| Abstract: |
| OBJECTIVE To study the transformation between oxymatrine and matrine, oxysophocarpine and sophocarpine in the extraction process of Sophora alopecuroides. METHODS Different extraction solvent (water and 55% ethanol), pH and extraction time were used to study the transformation rule of oxymatrine and matrine, oxysophocarpine and sophocarpine in Sophora alopecuroides Semen and Sophora alopecuroides herba by HPLC. RESULTS With the increasing of extraction time, the amount of oxidized alkaloids transformed into reduced alkaloids increased; extraction solvent made no difference in the effect of the transformation of Sophora alopecuroides alkaloids; with the increasing of extraction time, the additive amount of oxymatrine and matrine decreased slowly, the additive amount of oxysophocarpine and sophocarpine also decreased slowly. CONCLUSION The results provide a basis for quality control of alkaloids of Sophora alopecuroides, also can provide a basis for industrial extraction of Sophora alopecuroides. |
| Key words: matrine oxymatrine sophocarpine oxysophocarpine transformation |
