| 摘要: |
| 目的 建立同时测定玉叶金花清热片中栀子苷、穿心莲内酯和脱水穿心莲内酯含量的HPLC。方法 采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×150 mm，5 μm )，流动相为甲醇-0.2%磷酸水溶液，梯度洗脱，流速为1.0 mL?min-1，检测波长：238 nm(0~10 min，栀子苷)，225 nm(10~22 min，穿心莲内酯)，254 nm (22~35 min)。结果 栀子苷在0.166~2.981 μg内线性关系良好(r=0.999 9)，平均回收率为99.4%(RSD=1.2%)；穿心莲内酯在0.146~2.621 μg内线性关系良好(r=0. 999 9，平均回收率为99.2%(RSD=1.3%)；脱水穿心莲内酯在0.222~3.992 μg内性关系良好(r=0. 999 7)，平均回收率为100.1%(RSD=1.4%)。结论 该方法简便、快捷、准确，可用于该制剂的质量控制。 |
| 关键词: 玉叶金花清热片 栀子苷 穿心莲内酯 脱水穿心莲内酯 高效液相色谱法 |
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| Determination of Three Effective Components in Yuyejinhua Qingre Tablets by HPLC |
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TANG Dezhi
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Guangxi Nanning Institute for Food and Drug Control, Nanning 530001, China
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| Abstract: |
| OBJECTIVE To simultaneously determine geniposide, andrographolide and dehydroandrographolide in Yuyejinhua Qingre tablets by HPLC. METHODS Agilent ZORBAX SB-C18 (4.6 mm×150 mm, 5 μm ) column was used. The mobile phase was methanol-water containing 0.2% phosphoric acid with gradient elution mode at the flow rate of 1.0 mL?min-1; the detection wavelength was 238 nm(0-10 min) for geniposide, 225 nm(10-22 min) for andrographolide and 254 nm(22-35 min) for dehydroandrographolide. RESULTS Geniposide had a good linearity in the range of 0.166-2.981 μg, the average recovery was 99.4% with RSD of 1.2%. Andrographolide had a good linearity in the range of 0.146-2.621 μg, the average recovery was 99.2% with RSD of 1.3%. Dehydroandrographolide had a good linearity in the range of 0.222-3.992 μg, the average recovery was 100.1% with RSD of 1.4%. CONCLUSION The method is simple, accurate and reproducible, and can be used for quality control of Yuyejinhua Qingre tablets. |
| Key words: Yuyejinhua Qingre tablets geniposide andrographolide dehydroandro grapholide HPLC |
