| 引用本文: | 王京霞,陈琳,陈芳,林燕飞,孙静芸.正交试验优选防感颗粒的提取工艺[J].中国现代应用药学,2015,32(4):428-432. |
| WANG Jingxia,CHEN Lin,CHEN Fang,LIN Yanfei,SUN Jingyun.Optimum Extracting Procedure of Fanggan Granules by Orthogonal Design[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(4):428-432. |
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| 摘要: |
| 目的 确立防感颗粒的最佳提取工艺。方法 应用正交试验和HPLC，以防感颗粒中R,S-告依春、白花前胡甲素、苦杏仁苷及甘草苷4种活性成分含量为指标，采用多指标综合评分法，确定防感颗粒的提取工艺。结果 防感颗粒最佳提取工艺为：药材加8倍量水，煎煮3次，每次1.5 h，乙醇浓度为60%。结论 优选出的工艺稳定可靠，可用于防感颗粒的提取。 |
| 关键词: 防感颗粒 R,S-告依春 苦杏仁苷 白花前胡甲素 甘草苷 正交试验 提取工艺 |
| DOI： |
| 分类号:R283.6 |
| 基金项目: |
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| Optimum Extracting Procedure of Fanggan Granules by Orthogonal Design |
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WANG Jingxia1, CHEN Lin1, CHEN Fang1, LIN Yanfei2, SUN Jingyun2
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1.Zhejiang Hospital of Traditional Chinese Medicine, Hangzhou 310006, China;2.Hangzhou Innovation TCM Standardization Research Institute Co., Ltd., Hangzhou 310053, China
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| Abstract: |
| OBJECTIVE To establish the optimum extracting procedure of Fanggan granules. METHODS A multi index comprehensive evaluation method by orthogonal test and HPLC was used for the determination of the extracting procedure of Fanggan granules, with four active ingredients including (R,S)-epigoitrin, praeruptorin A, amygdalin and liquiritin as the content index. RESULTS The optimum extraction process of Fanggan granules was determined as follows: herbs were extracted 3 times with 8 times of water; 1.5 h each time; ethanol concentration was 60%. CONCLUSION The optimum procedure is stable and reliable for the extraction of Fanggan granules. |
| Key words: Fanggan granules (R,S)-epigoitrin amygdalin praeruptorin A liquiritin orthogonal design extracting procedure |
