| 引用本文: | 李莉,王庆振,孙颖平.近红外漫反射光谱结合主成分回归法快速测定黑果小檗皮中盐酸小檗碱含量[J].中国现代应用药学,2015,32(3):309-314. |
| LI Li,WANG Qingzhen,SUN Yingping.Near Infrared Diffuse Reflection Optical Spectra Combined with PCR Rapid Determination Content of Berberine Hydrochloride in Berberis Heteropoda Cortex[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(3):309-314. |
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| 摘要: |
| 目的 应用近红外漫反射光谱结合主成分回归法(PCR)对黑果小檗皮中盐酸小檗碱进行快速定量分析。方法 通过化学测定值与光谱间建立PCR数学模型对黑果小檗皮中盐酸小檗碱进行快速定量分析。结果 黑果小檗皮中盐酸小檗碱的内部交叉验证均方差(RMSECV)为1.016 9，内部交叉验证决定系数为0.979 5，预测平均回收率为108.51%，统计学检验表明预测值与标准方法测定值之间的差异无统计学意义。结论 表明利用近红外漫反射光纤光谱结合PCR对黑果小檗进行定量分析可行。 |
| 关键词: 黑果小檗 近红外光谱分析技术 主成分回归法 |
| DOI： |
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| 基金项目:国家自然科学基金(81260485) |
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| Near Infrared Diffuse Reflection Optical Spectra Combined with PCR Rapid Determination Content of Berberine Hydrochloride in Berberis Heteropoda Cortex |
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LI Li, WANG Qingzhen, SUN Yingping
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Xinjiang Medical University, Urumqi 830054, China
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| Abstract: |
| OBJECTIVE To analysis berberine hydrochloride in Berberis heteropoda cortex by frared diffuse reflection optical spectra combined with PCR. METHODS Between the measured value and chemical spectroscopy, a method of NIR diffuse reflectance optic-fiber spectroscopy combined with PCR was established to analysis berberine hydrochloride in Berberis heteropoda cortex. RESULTS The root-mean-square error of cross-validation(RMSECV) of the berberine hydrochloride in Berberis heteropoda cortex was 1.016 9, the correlation coefficients was 0.979 5, the average recycle rate was 108.5%. Statistical test was used to analysis the dates and it showed that the difference between NIR prediction values and the values determinated by standard methods had no statistical significance. CONCLUSION It indicates that NIR diffuse reflectance optic-fiber spectroscopy combined with PCR method can be used in rapid determination of the contents of Berberis heteropoda Schrenk. |
| Key words: Berberis heteropoda Schrenk near infrared reflectance spectroscopy(NIRS) PCR |
