| 引用本文: | 王莹,王乐秋,何志鹏,李丽.三氧化二砷诱导乳腺癌细胞凋亡的早期事件——凋亡性容量下降[J].中国现代应用药学,2015,32(3):274-277. |
| WANG Ying,WANG Leqiu,HE Zhipeng,LI Li.Apoptotic Volume Decrease is the Early Event in Arsenic Trioxide Induced Apoptosis in Breast Cancer Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(3):274-277. |
|
| 摘要: |
| 目的 探讨凋亡性容量下降(apoptotic volume decrease，AVD)是否是三氧化二砷(arsenic trioxide，As2O3)诱导乳腺癌MCF-7细胞凋亡的早期事件。方法 噻唑兰(MTT)法检测As2O3对MCF-7细胞存活率的影响；流式细胞技术检测As2O3能否引起MCF-7细胞凋亡；时滞显微摄影技术检测细胞容量；Caspase-3试剂盒检测凋亡信号活性。结果 MTT实验表明As2O3使MCF-7细胞存活率下降并呈现剂量依赖的量效关系；流式细胞测定结果证实As2O3对乳腺癌细胞的致凋亡作用；As2O3作用MCF-7细胞在2 h内细胞出现AVD，但Caspase-3的活性无明显变化，直到作用MCF-7细胞6 h后Caspase-3活性显著升高。结论 AVD是As2O3诱导乳腺癌细胞凋亡的早期事件。 |
| 关键词: 三氧化二砷 乳腺癌细胞 凋亡性容量下降 |
| DOI： |
| 分类号:R730.5 |
| 基金项目:牡丹江医学院科学技术研究项目(2011-07) |
|
| Apoptotic Volume Decrease is the Early Event in Arsenic Trioxide Induced Apoptosis in Breast Cancer Cells |
|
WANG Ying1, WANG Leqiu2, HE Zhipeng1, LI Li1
|
|
1.Department of Medical Function Research, Mudanjiang Medical University, Mudanjiang 157011, China;2.Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang 157011, China
|
| Abstract: |
| OBJECTIVE To investigate whether the apoptotic volume decrease is the early event in arsenic trioxide induced apoptosis in breast cancer MCF-7 cells. METHODS The effect of As2O3 on cell viability was assayed by methylthiazoletetrazolium(MTT). Apoptosis was analyzed by flow cytometry. Lag-time microphotography was employed to detect cell volume. The apoptotic signaling activity of Caspase-3 was detected by caspase assay kit. RESULTS MTT result showed As2O3 reduced the viability of MCF-7 cells in a concentration-dependent manner. Flow cytometry assay confirmed apoptosis resulted in As2O3-treated MCF-7 cells. Apoptotic volume decrease was observed within 2 h in As2O3 treated MCF-7 cells, but the caspase-3 activity had no significant change. The caspase-3 activity increased significantly until As2O3 treated MCF-7 cells for 6 h. CONCLUSION Apoptotic volume decrease is the early event in arsenic trioxide induced apoptosis in breast cancer cells. |
| Key words: arsenic trioxide MCF-7 cells apoptotic volume decrease |
