| 引用本文: | 陈璇,童晔玲,任泽明,杨锋,戴关海.杨梅酮对人肺腺癌A549细胞凋亡和细胞周期的影响[J].中国现代应用药学,2015,32(1):14-17. |
| CHEN Xuan,TONG Yeling,REN Zeming,YANG Feng,DAI Guanhai.Effect of Myricanone on Cell Apoptosis and Cell Cycle in Human Lung Adenocarcinoma A549 Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(1):14-17. |
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| 摘要: |
| 目的 检测杨梅酮对体外人肺腺癌细胞A549细胞凋亡和细胞周期的影响。方法 采用流式细胞仪分别检测各组的细胞早期凋亡率和细胞周期百分率。结果 杨梅酮能显著增加A549细胞早期凋亡率和G0/G1期的细胞比例，并且细胞凋亡率与杨梅酮浓度具有较好的量效关系。结论 杨梅酮能显著增加体外人肺腺癌细胞A549细胞早期凋亡率，并可使A549细胞周期阻滞在G0/G1期。 |
| 关键词: 杨梅酮 A549细胞 细胞凋亡 细胞周期 |
| DOI： |
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| 基金项目:浙江省科技厅项目(2011F20009、2013F50020、2013F10001) |
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| Effect of Myricanone on Cell Apoptosis and Cell Cycle in Human Lung Adenocarcinoma A549 Cells |
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CHEN Xuan, TONG Yeling, REN Zeming, YANG Feng, DAI Guanhai
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Institute of Basic Medicine, Zhejiang Academy of TCM, Hangzhou 310007, China
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| Abstract: |
| OBJECTIVE To detect the effect of myricanone on cell apoptosis and cell cycle in human lung adenocarcinoma A549 cells. METHODS The early cell apoptosis rate and cell cycle percentage of each group were detected by flow cytomytry. RESULTS Myricanone induced early cell apoptosis of A549 cells in a dose-dependent manner, as well as increased the percentage of A549 cells blocked in G0/G1 stage. CONCLUSION Myricanone could significantly increase the cell apoptosis rate of A549 cells and could block cell cycle of A549 cells in G0/G1 stage. |
| Key words: myricanone A549 cell cell apoptosis cell cycle |
