| 摘要: |
| 目的 建立同时测定百乐眠胶囊中2,3,5,4＇-四羟基二苯乙烯-2-O-β-D葡萄糖苷、远志口山酮Ⅲ、3,6二芥子酰基蔗糖的高效液相色谱法。方法 采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×250 mm，5 mm)；流动相：甲醇-乙腈-0.05%磷酸溶液(32∶8∶60)；流速：1.0 mL·min-1；检测波长：320 nm。结果 2,3,5,4＇-四羟基二苯乙烯-2-O-β-D葡萄糖苷、远志口山酮Ⅲ和3,6二芥子酰基蔗糖线性范围分别为0.020 6~0.309 mg，0.041 6~0.624 mg，0.022 4~0.336 mg，线性关系良好(r依次为0.999 6，0.999 4，0.999 7)，平均回收率分别为94.63%，99.63%，98.11%(n=9)，RSD分别为2.1%，1.1%，1.0%。结论 该方法准确可靠，重复性好，为百乐眠胶囊的质量控制提供了依据。 |
| 关键词: 百乐眠胶囊 2,3,5,4＇-四羟基二苯乙烯-2-O-β-D葡萄糖苷 远志口山酮Ⅲ 3,6二芥子酰基蔗糖 高效液相色谱法 含量测定 |
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| Simultaneous Determination of Three Components in Bai Lemian Capsule by HPLC |
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FU Suhua, LYU Linfeng
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Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China
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| Abstract: |
| OBJECTIVE To establish a method for determination of 2,3,5,4＇-tetrahydroxy stilbene-2-Ο-β-D-glucoside, polygalaxanthone III and 3,6-disinapoyl sucrose in Bai Lemian capsule by HPLC. METHODS An Agilent Eclipse XDB-C18 column(4.6 mm×250 mm, 5 mm) was used. The mobile phase was methyl alcohol-acetonitrile-0.05% phosphoric acid solution (32∶8∶60), the flow rate was 1.0 mL·min-1, and the detection wavelength was 320 nm. RESULTS The linear range for 2,3,5,4＇-tetrahydroxy stilbene-2-Ο-β-D-glucoside, polygalaxanthone Ⅲ and 3,6-disinapoyl sucrose were 0.020 6-0.309 mg, 0.041 6-0.624 mg and 0.22 4-0.336 mg(r=0.999 6, 0.999 4, 0.999 7), the average recovery were 94.63%, 99.63%, 98.11%(n=9), and RSD were 2.1%, 1.1%, 1.0%, respectively. CONCLUSION The method is accurate, credible and good reproducible. It can be used for the quality control of Bai Lemian capsule. |
| Key words: Bai Lemian capsule 2,3,5,4＇-tetrahydroxy stilbene-2-Ο-β-D-glucoside polygalaxanthone III 3,6-disinapoyl sucrose HPLC determination |
