| 引用本文: | 叶强,睢凤英,杨鑫骥,王嘉.抗癌灵活性部位对不同肿瘤细胞的抑制作用[J].中国现代应用药学,2014,31(5):534-536. |
| YE Qiang,SUI Fengying,YANG Xingji,WANG Jia.Anti-tumor Effect of Kang Ai Ling’s Active Site on Different Cancer Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(5):534-536. |
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| 摘要: |
| 目的 研究抗癌灵正丁醇提取部位(以下简称KAL)对多种肿瘤细胞的抑制率以及时效和量效关系。方法 采用MTT法考察KAL对肿瘤细胞B-16、SGC-7901、Colo320、PC-3、HL60的抑制率以及IC50；通过观察KAL对肿瘤细胞SGC-7901和PC-3生长的影响，考察其时效和量效关系。结果 KAL对肿瘤细胞B-16、SGC-7901、Colo320、PC-3、HL60的IC50分别为：(21.05±4.31)，(13.43±4.78)，(15.17±3.82)，(26.39±5.76)，(25.78±4.40)μg·mL-1，且在抑制肿瘤细胞过程中呈现明显的时效关系和量效关系。结论 KAL对肿瘤细胞B-16、SGC-7901、Colo320、PC-3、HL60具有显著抑制作用。 |
| 关键词: 抗癌灵 正丁醇提取部位 抗肿瘤 |
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| 基金项目:浙江医学高等专科学校科研基金项目(2006XZ11) |
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| Anti-tumor Effect of Kang Ai Ling’s Active Site on Different Cancer Cells |
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YE Qiang1, SUI Fengying2, YANG Xingji2, WANG Jia3
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1.Zhejiang Provincial People’s Hospital, Hangzhou 310014, China;2.Jiaxing University, College of Medicine, Jiaxing 314000, China;3.Zhejiang Medical College, Hangzhou 310053, China
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| Abstract: |
| OBJECTIVE To observe the growth inhibitory effect of tumor cells by Kang Ai Ling’s 1-butanol exrtract(KAL). METHODS The anti-proliferative activities of 1-butanol extract of KAL against B-16, SGC-7901, Colo320, PC-3 and HL60 cells were evaluated by MTT colorimetric assay in vitro, then the result showed by inhibitory rate and IC50. Investigation on KAL’s dose-effect dependent manner and time-effect dependent manner through growth inhibitory curve on SGC-7901 and PC-3. RESULTS The IC50 of KAL on B-16, SGC-7901, Colo320, PC-3 and HL60 were (21.05±4.31), (13.43±4.78), (15.17±3.82), (26.39±5.76), (25.78±4.40)μg·mL-1, respectively. The anti-tumor effect of KAL has obvious time-effect and dose-effect dependence. CONCLUSION KAL has significant growth inhibitory effect on B-16, SGC-7901, Colo320, PC-3 and HL60 cells. |
| Key words: Kang Ai Ling 1-butanol extract anti-tumor |
