| 引用本文: | 游隽,蔡盛,吴淑萍.β-环糊精手性流动相添加剂法测定爱维莫潘中的异构体[J].中国现代应用药学,2014,31(9):1101-1104. |
| YOU Jun,CAI Sheng,WU Shuping.Separation of Alvimopan and Its Enantiomers by HPLC Using β-cyclodextrin as Chiral Mobile Phase Additive[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(9):1101-1104. |
|
| 摘要: |
| 目的 建立爱维莫潘的高效液相手性拆分分析法。方法 选用C18柱(4.6 mm×250 mm，5 μm)，流动相为水溶液(含0.5%冰醋酸，0.5%三乙胺，1%β-环糊精)-甲醇(80∶20)，柱温为40 ℃，检测波长为275 nm。结果 爱维莫潘和它的3个异构体都达到良好分离，分离度符合要求。结论 本方法具有专属性强，灵敏度高，重复性良好，耐用性好，简便快捷，使用成本低廉等优点。 |
| 关键词: β-环糊精 手性流动相添加剂 异构体分离 爱维莫潘 |
| DOI： |
| 分类号:R284.1；R917.101 |
| 基金项目: |
|
| Separation of Alvimopan and Its Enantiomers by HPLC Using β-cyclodextrin as Chiral Mobile Phase Additive |
|
YOU Jun1, CAI Sheng2, WU Shuping2
|
|
1.Zhejiang Cancer Hospital, Hangzhou 310022, China;2.Hangzhou SIMBOS Pharm Co., Ltd., HangZhou 310030, China
|
| Abstract: |
| OBJECTIVE To separate the enantiomers of alvimopan by HPLC which was developed using chiral mobile phase. METHODS Separation was performed on a C18 column(4.6 mm×250 mm, 5 μm). The mobile phase was methanol- water(concentration of 0.5% glacial acetic acid, 0.5% triethylamine and 1%β-cyclodextrin)(20∶80). The detection wavelength was set at 275 nm and the temperature was 40 ℃. RESULTS With this method, alvimopan and its enantiomers were well separated, the resolution met the requirement. CONCLUSION The method is simple, specific, sensitive, repeatable accurate and inexpensive. |
| Key words: β-cyclodextrin chiral mobile phase additive enantiomeric resolution alvimopan |
