| 引用本文: | 王芳,陈翔.HPLC测定感冒清胶囊中对乙酰氨基酚和盐酸吗啉胍的溶出度[J].中国现代应用药学,2014,31(10):1245-1248. |
| WANG Fang,CHEN Xiang.Dissolution Determination of Acetaminophen and Moroxydine Hydrochloride in Ganmaoqing Capsule by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(10):1245-1248. |
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| 摘要: |
| 目的 建立感冒清胶囊中对乙酰氨基酚和盐酸吗啉胍的溶出度测定方法。方法 采用桨法，以0.1 mol·L-1的盐酸溶液为溶出介质，转速为50 r·min-1，取样时间为30 min。高效液相色谱法采用 ZORBAX SB-C18(150 mm×4.6 mm，5 μm)色谱柱，以乙腈-0.02 mol·L-1庚烷磺酸钠溶液(15∶85)为流动相，检测波长为237 nm，流速为1.0 mL·min-1，柱温为30 ℃。结果 对乙酰氨基酚在4.588×10-2~0.458 8 μg内呈良好的线性关系(r=0.999 8)，平均回收率为98.1%，RSD为1.06%(n=9)；盐酸吗啉胍在4.654×10-2~0.465 4 μg内呈良好的线性关系(r=0.999 6)，平均回收率为97.9%，RSD为1.09%(n=9)。结论 该法简便、准确、可行，能准确测定感冒清胶囊中对乙酰氨基酚和盐酸吗啉胍的溶出度，专属性良好。 |
| 关键词: 感冒清胶囊 对乙酰氨基酚 盐酸吗啉胍 高效液相色谱法 |
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| Dissolution Determination of Acetaminophen and Moroxydine Hydrochloride in Ganmaoqing Capsule by HPLC |
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WANG Fang1, CHEN Xiang2
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1.Department of Pharmacy, Hospital of Zhoushan City, Zhoushan 316021, China;2.Zhoushan Municipal Institute for Food and Drug Control, Zhoushan 316021, China
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| Abstract: |
| OBJECTIVE To establish the method of dissolution determination of acetaminophen and moroxydine hydrochloride in Ganmaoqing capsule. METHODS Paddle apparatus was used with 0.1 mol·L-1 hydrochloric acid solution as the dissolution medium at a speed of 50 r·min-1 and dissolution time limit was 30 min. The analysis was carried out on a ZORBAX SB-C18(150 mm×4.6 mm, 5 μm) column, the mobile phase was composed of acetonitrile-0.02 mol·L-1 sodium heptanesulfonate(15∶85) at the flow rate of 1.0 mL·min-1 and the detection wavelength was 237 nm. RESULTS Acetamino- phen showed good linearity in the range of 4.588×10-2-0.458 8 μg(r=0.999 8), the average recovery was 98.1% with RSD of 1.06%(n=9); moroxydine hydrochloride showed good linearity at the range of 4.654×10-2-0.465 4 μg(r=0.999 6), the average recovery was 97.9% with RSD of 1.09%(n=9). CONCLUTION The method is convenient, accurate and practicable. It can be applied to determine the dissolution of the preparations. |
| Key words: Ganmaoqing capsule acetaminophen moroxydine hydrochloride HPLC |
