• 首页期刊简介编委会刊物订阅专栏专刊电子刊学术动态联系我们English
引用本文:郭培果.HPLC测定参茸珍宝片中特女贞苷的含量[J].中国现代应用药学,2014,31(10):1231-1234.
GUO Peiguo.Determination of Specnuezhenide in Shenrongzhenbao Tablets by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(10):1231-1234.
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 2378次   下载 1206 本文二维码信息
码上扫一扫!
分享到: 微信 更多
HPLC测定参茸珍宝片中特女贞苷的含量
郭培果
贵阳济仁堂药业有限公司,贵阳 550005
摘要:
目的 建立测定参茸珍宝片中特女贞苷含量的方法。方法 采用反相高效液相色谱法,色谱柱为Ultimate XB-C18-H (250 mm×4.6 mm,5 mm),流动相为甲醇-水(38∶62),流速为1.0 mL·min-1,检测波长为224 nm,进样量为10 μL。结果 特女贞苷进样量在0.053 44~1.603 3 mg内与峰面积积分值线性关系良好(r=0.999 9);平均加样回收率为96.90%,RSD为0.93%。结论 该方法快速简便、准确可靠、重复性好,可用于参茸珍宝片中特女贞苷的含量测定。
关键词:  参茸珍宝片  特女贞苷  高效液相色谱法  含量测定
DOI:
分类号:
基金项目:
Determination of Specnuezhenide in Shenrongzhenbao Tablets by HPLC
GUO Peiguo
Guiyang Jirentang Pharmaceutical Manufacture Co., Ltd., Guiyang 550005, China
Abstract:
OBJECTIVE To establish an HPLC method of specnuezhenide content in Shenrongzhenbao tablets. METHODS Separation was performed on a Ultimate XB-C18-H column with methanol-water(38∶62) as the mobile phase. The detection wavelength was 224 nm, the flow rate was 1.0 mL·min-1, and the injection volume was 10 μL. RESULTS In the range of 0.053 44~1.603 3 μg, the calibration curve showed good linearity(r=0.999 9). The average recovery of specnuezhenide was 96.90% and RSD was 0.93%. CONCLUSION The method is simple and reliable with good repeatability, which can be used for the determination of specnuezhenide in Shenrongzhenbao tablets.
Key words:  Shenrongzhenbao tablets  specnuezhenide  HPLC  content determination
扫一扫关注本刊微信