| 摘要: |
| 目的 建立检查中药洗剂中非法添加肾上腺皮质激素的LC-MS方法。方法 采用液相色谱-三重四极杆质谱联用仪同时检测中药洗剂中非法添加16种肾上腺皮质激素类化学成分。进行定性和定量分析，并进行方法学验证。结果 16种常被用于非法添加的肾上腺皮质激素类药物在色谱柱中得到了良好的分离，再配合产物离子质谱图进行定性，利用多反应监测模式的峰面积进行定量计算，专属性强，精密度良好，回收率和线性关系满足分析要求。结论 该方法简便、准确，可以用于中药洗剂中非法添加肾上腺皮质激素类化学成分的实验室筛查。 |
| 关键词: 中药洗剂 肾上腺皮质激素 液质联用 |
| DOI： |
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| Study on LC-MS Method for Detection of 16 Kinds of Adrenal Cortical Hormone Illegally Added in Chinese Medicinal Lotions |
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PAN Jun
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Binzhou Institute for Drug Control, Binzhou 256618, China
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| Abstract: |
| OBJECTIVE To establish a specialized detecting method for the adrenal cortical hormone added illegally in Chinese medicinal lotion. METHODS Used LC-MS/MS to simultaneous detect 16 components added illegally in Chinese medicinal lotion. Though qualitative and quantitative analysis, validated a methodology. RESULTS Each of the 16 chemical components separated in chromatographic column, at the same time, used the two grade mass spectrum for qualitative and the peak area of multiple reaction monitoring mode for quantitative calculation, it’s recovery and linear relationship met the demand of screening. CONCLUSION This method is simple and accurate to detect chemical components of the adrenal cortical hormone added illegally in Chinese medicinal lotion. |
| Key words: Chinese medicinal lotion adrenal cortical hormone LC-MS |
