| 引用本文: | 罗慧英,黄亚红,杨林.壳聚糖纳米颗粒对K562细胞增殖的影响[J].中国现代应用药学,2014,31(10):1175-1178. |
| LUO Huiying,HUANG Yahong,YANG Lin.Effect of Chitosan Nanoparticles on the Proliferation of K562 Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(10):1175-1178. |
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| 摘要: |
| 目的 评价壳聚糖纳米颗粒对K562细胞增殖的影响，并探讨相关机制。方法 采用MTT法对壳聚糖纳米颗粒的最佳作用时间和作用浓度进行筛选，免疫荧光技术和透射电镜技术考察壳聚糖纳米颗粒对K562细胞形态的影响，流式细胞仪检测壳聚糖纳米颗粒对线粒体膜电位、细胞内ROS含量和Ca2+浓度的影响。结果 壳聚糖纳米颗粒(15 μg·mL-1)可以明显诱导K562细胞出现凋亡改变，表现为：细胞核变小，出现核聚缩等现象。同时线粒体膜电位崩解，ROS含量增加，Ca2+浓度增加。结论 壳聚糖纳米颗粒对K562细胞的增殖有抑制作用，其机制可能与诱导细胞凋亡有关。 |
| 关键词: 壳聚糖纳米颗粒 K562细胞 增殖 细胞凋亡 |
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| 基金项目:甘肃省青年科技基金计划(1208RJYA024) |
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| Effect of Chitosan Nanoparticles on the Proliferation of K562 Cells |
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LUO Huiying1,2, HUANG Yahong1, YANG Lin1
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1.Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China;2.Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicine of Gansu Province, Lanzhou 730000, China
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| Abstract: |
| OBJECTIVE To evaluate the effect of chitosan nanoparticles on the proliferation of K562 cells, and to explore the related mechanism. METHODS The best time and concentration of chitosan nanoparticles were screened by MTT method. Immunofluorescence and transmission electron microscope were used to study the effect of chitosan nanoparticles on the morphology of K562 cells. FACS was used to detect the effect of chitosan nanoparticles on MMP, intracellular ROS concentration, and Ca2+ concentration. RESULTS Chitosan nanoparticles(15 μg·mL-1) could induce K562 cells apoptosis, such as nuclei smaller, nuclear condensation phenomena, company with the collapse of mitochondrial membrane potential, increased ROS content, and the increased of Ca2+ concentration. CONCLUSION Chitosan nanoparticles can inhibit the proliferation of K562 cells, the mechanism may be related to inducing cell apoptosis. |
| Key words: chitosan nanoparticles K562 cells proliferation apoptosis |
