| 摘要: |
| 目的 采用热水浸提柚皮中柚皮苷，并使用大孔吸附树脂对其进一步富集，并考察其活性。方法 以柚皮苷的吸附率和解吸率为考察指标，筛选不同大孔吸附树脂对柚皮苷的吸附性能和洗脱能力，并初步考察柚皮苷抗氧化作用和镇静作用。结果 大孔树脂HPD450有较好的吸附效果，工艺条件：上样流速为2 BV·h-1，样液浓度为2.36 mg·mL-1；解吸最适工艺条件：洗脱剂乙醇浓度为50%，用量为4 BV，流速为3 BV·h-1。浓度为3.75 mg·mL-1的柚皮苷具有较好的抗氧化作用，以20 mg·kg-1给药时，小白鼠进入睡眠时间较短且行动比较缓慢。结论 HPD450可用于富集柚皮中的柚皮苷，得到的产物具有较好的镇静和安眠作用。 |
| 关键词: 文旦柚 柚皮苷 富集 抗氧化 镇静 |
| DOI： |
| 分类号: |
| 基金项目:福建省高校服务海西建设重点项目(2008HX01) |
|
| Study on Enrichment and Function of Naringin from Shaddock Peel |
|
LIN Guorong
|
|
Putian University College of Environmental and Biological Engineering, Putian 351100, China
|
| Abstract: |
| OBJECTIVE To extract naringin from pomelo with hot water and enriched by macroporous resin, and then observe its function. METHODS The adsorption and elution performances of various macroporous resins were screened using the adsorption rate and desorption rate as indexes. RESULTS HPD450 had the best adsorption effect. The optimal adsorption conditions were 2 BV·h-1 of upflow rate and 2.36 mg·mL-1 of concentration as well as the optimal desorption conditions were 50% of ethanol concentration, 4 BV of dosage and 3 BV·h-1 of flow rate. The preliminary function study indicated that naringin had a sound anti-oxidation performance at a concentration of 3.75 mg·mL-1. In addition, the mice presented less sleep latencies and moved relative slowly at a dosage of 20 mg·kg-1 of naringin. CONCLUSION HPD450 has the good adsorption edffect, suggesting that naringin has good sedative and hypnotic effects. |
| Key words: shaddock naringin enrichment antioxidant sedation |
