| 引用本文: | 邓思珊,刘洪旭,王全,王伟峰,梁一池.不同产地草珊瑚优良种源筛选研究[J].中国现代应用药学,2014,31(9):1061-1066. |
| DEND Sishan,LIU Hongxu,WANG Quan,WANG Weifeng,LIANG Yichi.Study on Screening of High Quality Sarcandra Glabra in Different Provenance[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(9):1061-1066. |
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| 摘要: |
| 目的 测定46个不同产地种源草珊瑚叶、茎的生物量以及活性成分落新妇苷、迷迭香酸、异嗪皮啶含量，以总量为考查指标，筛选优良种源。方法 样品来自福建三明三元吉口采育场草珊瑚种植栽培示范区3年生成熟草珊瑚，每个产地随机取20株，分叶与茎，晾干，称量，计算成每10株叶、茎干品重量作为生物量；含量测定采用HPLC。色谱柱：Ultimate C18(250 mm×4.6 mm，5 μm)；流动相A为乙腈-0.2%磷酸溶液(18∶82)，B为乙腈-0.2%磷酸溶液(30∶70)；梯度洗脱：0~30 min，A→B；流速：1 mL·min-1；柱温：室温；检测波长：茎部位(测定迷迭香酸、异嗪皮啶)为344 nm，叶部位(测定落新妇苷、迷迭香酸)为290 nm；分析有效成分含量、生物量以及总量。结果 不同地理种源草珊瑚生物量差别显著，含量差别较大，计算总含量与总量均有较大差异，筛选5个高产质优的产地有3个来自福建三明。结论 多指标有效成分总含量结合生物量筛选高产优质良种，指导科学种植有很大意义。 |
| 关键词: 草珊瑚 生物量 高效液相色谱法 迷迭香酸 落新妇苷 异嗪皮啶 |
| DOI： |
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| 基金项目:福建省自然科学基金项目(2013J01366) |
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| Study on Screening of High Quality Sarcandra Glabra in Different Provenance |
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DEND Sishan1, LIU Hongxu1, WANG Quan2, WANG Weifeng3, LIANG Yichi3
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1.Fujian Academy of Medical Sciences, Fujian Key Laboratory of Medical Testing, Fuzhou 350001, China;2.Fujian Medical University, Fuzhou 350108, China;3.Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China
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| Abstract: |
| OBJECTIVE To screen high quality Sarcandra glabra by determine biomasses and active components of astilbin, rosmarinic acid and isofraxidin in leaves and stems from 46 provenances. METHODS Fourty-six samples of different geographic provenance Sarcandra glabra were collected from Fujian Sanming cultivation base of the nation’s largest planting scale, random sampling of 20 strains of above ground part, separate leaves and stems, dried, weighed, calculated as per 10 of leaves, stems weight as biomasses. Determined content by HPLC. Chromatographic column: Ultimate C18(250 mm×4.6 mm, 5 μm); mobile phase: A: acetonitrile-0.2% phosphoric acid(18∶82), B: acetonitrile-0.2% phosphoric acid (30∶70), 0~30 min, A→B gradient elution; flow rate: 1 mL·min-1; column temperature: room temperature; detection wavelength: stems(determination of rosmarinic acid, isofraxidin) was 344 nm, and leaves(determination of astilbin, rosmarinic acid) was 290 nm; analysis of biomass and total content of effective components. RESULTS Different provenances of Sarcandra glabra biomasses were significantly different, contents of effective components were greatly different, the total content were quite different, the 5 screening high quality provenances had 3 from Fujian Sanming. CONCLUSION The screening of high-quality multi index combined with effective component of total content of biomasses, has great significance to guide scientific planting. |
| Key words: Sarcandra glabra biomass HPLC rosmarinic acid astilbin isofraxidin |
