| 引用本文: | 肖功胜,王永兵,雷辉,宗伟英,沈雯,王滔,席晓荣.不同产地白薇中C21甾体皂苷的含量测定[J].中国现代应用药学,2014,31(10):1228-1231. |
| XIAO Gongsheng,WANG Yongbing,LEI Hui,ZONG Weiying,SHEN Wen,WANG Tao,XI Xiaorong.Determination of C21-Steroidal-glycoside in Cynanchi Atrati Radix from Different Areas[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(10):1228-1231. |
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| 摘要: |
| 目的 建立白薇中蔓生白薇苷A及白薇C21甾体总皂苷的含量测定方法，并测定其在不同产地蔓生白薇中的含量。方法 以蔓生白薇苷A为指标，采用高效液相色谱法和可见分光光度法分别对蔓生白薇苷A及白薇C21甾体总皂苷进行含量测定。结果 蔓生白薇苷A和总皂苷含量分别在0.23~2.53 μg(r=0.999 7)及11.5~115 μg(r=0.999 1)内线性关系良好，平均回收率分别为99.3%(RSD=2.68%)和99.7%(RSD=2.84%)。蔓生白薇苷A的含量以河南最高，山东最低；白薇C21甾体总皂苷则以山东最高，辽宁最低。结论 此方法简便、稳定可靠，便于对白薇药材进行质量评价。 |
| 关键词: 白薇 C21甾体皂苷 蔓生白薇皂苷A 含量测定 |
| DOI： |
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| 基金项目:湖州市自然科学资金项目(2013YZ13) |
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| Determination of C21-Steroidal-glycoside in Cynanchi Atrati Radix from Different Areas |
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XIAO Gongsheng, WANG Yongbing, LEI Hui, ZONG Weiying, SHEN Wen, WANG Tao, XI Xiaorong
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Huzhou Research and Development Center for Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Huzhou 313000, China
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| Abstract: |
| OBJECTIVE To establish the method for the determination of cynanversicoside A and total C21- steroidal-glycoside in Cynanchi Atrati Radix from different areas. METHODS With the content and absorbance of cynanversicoside A as index, HPLC and UV were used to determine the contents of cynanversicoside A and total C21-steroidal-glycoside in all samples. RESULTS The linear range of cynanversicoside A and steroidal-glycoside was 0.23-2.53 μg and 11.5-115 μg, respectively, with the correlation coefficient of 0.999 7 and 0.999 1; the average recoveries were 99.3% and 99.7%, with RSD of 2.68% and 2.84%. There was great difference of the samples, content of cynanversicoside A of Henan was the highest, lowest in Shandong; the total C21-steroidal-glycoside in sample from Shandong was the highest, lowest in Liaoning. CONCLUSION The method is simple, accurate and reliable for the quality control of Cynanchi Atrati Radix. |
| Key words: Cynanchi Atrati Radix C21-steroidal-glycoside cynanversicoside A content determination |
