| 引用本文: | 徐爱仁,应景艳,何文跃,陈武,马卫成.载多柔比星二氧化钛纳米粒的制备及体外评价[J].中国现代应用药学,2014,31(8):962-965. |
| XU Airen,YING Jingyan,HE Wenyue,CHEN Wu,MA Weicheng.Preparation and Evaluation of Doxorubicin-loaded Titanium Dioxide Nanoparticles[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(8):962-965. |
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| 摘要: |
| 目的 制备载多柔比星(doxorubicin，DOX)的二氧化钛(TiO2)纳米粒，并考察其体外释放百分率及细胞毒性。方法 通过水热法合成DOX的TiO2纳米粒，采用透射电镜及X-射线衍射仪对其进行表征，紫外可见分光光度法测定载药量及体外释放，采用MTT法分析其对MCF-7细胞和Hela细胞的细胞毒性。结果 所制备的纳米粒分散均匀，外观呈梭状，长度约为200 nm，在水中的载药量达10.85%，体外释放具有pH敏感性，空白纳米粒细胞毒性较低，载药纳米粒的细胞毒性与游离多柔比星相当。结论 所制备的TiO2纳米粒具有较高的载药量及pH敏感的体外释放性能，可作为DOX的载体。 |
| 关键词: 细胞毒性 二氧化钛 多柔比星 pH敏感释放 |
| DOI： |
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| 基金项目:宁波市自然科学基金项目(2012A610182) |
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| Preparation and Evaluation of Doxorubicin-loaded Titanium Dioxide Nanoparticles |
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XU Airen, YING Jingyan, HE Wenyue, CHEN Wu, MA Weicheng
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Department of Pharmacy, Renopathy and Urology Hospital, Ningbo 315100, China
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| Abstract: |
| OBJECTIVE To prepare doxorubicin(DOX)-loaded titanium dioxide(TiO2) nanoparticles and evaluate in vitro release and cytotoxicity. METHODS The TiO2 nanoparticles were sythesized using solvothermal reaction and then characterized with TEM and X-ray diffraction (XRD). The drug loading capacity and release profile were determined using UV-Vis spectrophotometer. The cytotoxicity against MCF-7 and Hela cells was evaluated by MTT method. RESULTS The obtained TiO2 nanoparticles with the length about 200 nm were well dispersed in water. The drug loading capacity in water was 10.85%. The release of DOX in vitro was pH sensitive. No obvious cytotoxicity of TiO2 nanoparticles was observed. The drug-loaded nanoparticles showed equivalent cytotoxicity against MCF-7 and Hela cells with free DOX. CONCLUSION TiO2 nanoparticles which show high drug loading capacity and controllable drug release can be used as a novel carrier for the antitumor drug doxorubicin. |
| Key words: cytotoxicity titanium dioxide(TiO2) doxorubicin pH sensitive release |
