| 引用本文: | 梁晶,胡卢丰,林观样,金辉.顺铂对阿霉素与人血清白蛋白结合的相互作用光谱研究[J].中国现代应用药学,2014,31(7):832-835. |
| LIANG Jing,HU Lufeng,LIN Guanyang,JIN Hui.Spectroscopy Study of Cisplatin and Adriamycin on Human Serum Albumin Interactions[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(7):832-835. |
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| 摘要: |
| 目的 研究顺铂对阿霉素与人血清白蛋白结合的相互作用。方法 采用荧光光谱法研究不同浓度顺铂对阿霉素与人血清白蛋白结合的相互作用。结果 顺铂与阿霉素对人血清白蛋白都有猝灭作用。顺铂对白蛋白的猝灭方式为动态猝灭，而阿霉素对白蛋白的猝灭方式为静态猝灭。结论 温度为17 ℃和37 ℃时，阿霉素与白蛋白的结合常数分别为2.13×104，2.76×104 L·mol-1，2者的结合位点数为1。当加入不同浓度的顺铂后，阿霉素与白蛋白的结合常数有所变化，而结合位点数仍然为1。 |
| 关键词: 顺铂 阿霉素 人血白蛋白 相互作用 光谱研究 |
| DOI： |
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| 基金项目:温州市科技计划项目(S20100050) |
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| Spectroscopy Study of Cisplatin and Adriamycin on Human Serum Albumin Interactions |
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LIANG Jing, HU Lufeng, LIN Guanyang, JIN Hui
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Department of Pharmacy, the Frist Affilated Hospital of Wenzhou Medical College, Wenzhou 325000, China
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| Abstract: |
| OBJECTIVE To study cisplatin and adriamycin on human serum albumin(HSA) interactions. METHODS The interaction of cisplatin and adriamycin on HSA was studied by fluorescence spectra. RESULTS The results showed that cisplatin and adriamycin strongly quenched the fluorescence of human serum albumin. The quenching mevhanisn of cisplatin was a dynamic quenching procedure, and the quenching mevhanisn of adriamycin was a static quenching procedure. CONCLUSION At 17 ℃ and 37 ℃, the binding constant of adriamycin and HSA system are 2.13×104 and 2.76×104 L·mol-1, furthermore, the binding is 1. When different concentrations of cisplatin is add to the adriamycin and HSA system, the binding constant changed, and the binding site is still 1. |
| Key words: cisplatin adriamycin human serum albumin interaction spectroscopy study |
