| 引用本文: | 颜琳琦,戚绿叶,张丽媛,陶巧凤.HPLC测定保健食品中微胶囊型维生素A[J].中国现代应用药学,2014,31(7):860-863. |
| YAN Linqi,QI Lüye,ZHANG Liyuan,TAO Qiaofeng.Determination of Vitamin A in the Form of Microcapsule in Functional Food by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(7):860-863. |
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| 摘要: |
| 目的 建立测定保健食品中微胶囊型维生素A含量的反相高效液相色谱法。方法 以适宜浓度氨水溶液破坏保健食品中微胶囊型维生素A的微胶囊结构，再以有机溶剂提取。采用Agilent ZORBAX SB-C18色谱柱(250 mm×4.6 mm，5 μm)，以甲醇为流动相，流速1.0 mL·min-1，检测波长为325 nm，柱温35 ℃。结果 维生素A在1.41~56.4 μg·mL-1内线性关系良好(r=1.000)；平均回收率为97.5%(RSD=1.9%)。结论 本法方法简单、准确，重现性好，适用于保健食品中微胶囊型维生素A的测定。 |
| 关键词: 高效液相色谱 维生素A 微胶囊 保健食品 |
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| Determination of Vitamin A in the Form of Microcapsule in Functional Food by HPLC |
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YAN Linqi1, QI Lüye2, ZHANG Liyuan1, TAO Qiaofeng1
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1.Zhejiang Institute for Food and Drug Control, Hangzhou 310004, China;2.College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China
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| Abstract: |
| OBJECTIVE To develop an HPLC method for determination of vitamin A in the form of microcapsule in functional food. METHODS Microcapsule structure was disrupted by suitable ammonia solution and vitamin A was extracted by organic solutions. The chromatographic separation was achieved on an Agilent ZORBAX SB-C18 column(250 mm×4.6 mm, 5 μm) with methanol as the mobile phase. The detection wavelength was at 325 nm, flow rate 1.0 mL·min-1 and the column temperature 35 ℃. RESULTS The linear range of vitamin A was 1.41-56.4 μg·mL-1(r=1.000). The average recovery of vitamin A was 97.5%(RSD=1.9%). CONCLUSION The established method was simple, accurate and with good reproducibility, suitable for content determination of vitamin A in functional food. |
| Key words: HPLC vitamin A microcapsule functional food |
