| 引用本文: | 吕宏迪,马珍珍,郝少君,孙建华,张正臣.心脑宁片对脑缺血大鼠乳酸、LDH、ATP酶活力的影响[J].中国现代应用药学,2014,31(9):1058-1061. |
| LYU Hongdi,MA Zhenzhen,HAO Shaojun,SUN Jianhua,ZHANG Zhengchen.Effect of Xinnaoning Tablets on Lactic Acid, LDH and ATP in Cerebral Ischemia Rats[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(9):1058-1061. |
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| 摘要: |
| 目的 研究心脑宁片对大鼠脑缺血模型脑匀浆乳酸(lactic acid，LD)、乳酸脱氢酶(lactate dehydrogenase，LDH)水平及三磷酸腺苷(adenosine-triphosphate，ATP)酶活力的影响。方法 Wistar大鼠随机分为7组，每组12只：空白对照组，高、中、低剂量心脑宁片组、尼莫地平组、脑安片组和羧甲基纤维素钠组，测定脑匀浆中蛋白含量、LD、LDH、ATP酶水平。结果 大鼠脑缺血模型造模成功。与模型组相比，各剂量心脑宁片均可显著降低脑匀浆LD水平(P<0.01)，升高脑匀浆LDH活力(P<0.01)，并可显著升高脑匀浆Na+-K+-ATPase(P<0.01)、Mg2+-ATPase和Ca2+-ATPase活力(P<0.01或P<0.05)结论 心脑宁片具有明显改善脑缺血作用。 |
| 关键词: 心脑宁片 大鼠脑缺血模型 乳酸 乳酸脱氢酶 ATP酶活力 |
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| 基金项目:]河南省科学技术厅2009年科技发展计划,项目编号：092102310202专利号ZL2011 1 0462034.9 |
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| Effect of Xinnaoning Tablets on Lactic Acid, LDH and ATP in Cerebral Ischemia Rats |
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LYU Hongdi, MA Zhenzhen, HAO Shaojun, SUN Jianhua, ZHANG Zhengchen
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The 371th Central Hospital of PLA, Xinxiang 453000, China
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| Abstract: |
| OBJECTIVE To study the effect of Xinnaoning tablets on lactic acid, LDH and ATPase activity effect in cerebral ischemia rats. METHODS Wistar rats were randomly divided into 7 groups: control group, model, high- middle- and low-dose of Xinnaoning tablets group, nimodipine group, Naoan tablets group and 0.5% CMC group. The content of brain homogenate protein, LD, LDH, ATPase levels were determined. RESULTS The model of cerebral ischemia rats were established successfully. Compared with model group, high-, middle-, and low- dose of Xinnaoning tablets could significantly decrease LD, raise LDH, and elevate brain homogenates of Na+-K+-ATPase, Mg2+-ATPase activity and Ca2+-ATPase activity (P<0.01 or P<0.05). CONCLUSION Xinnaoning tablets have significantly improve effect on cerebral ischemia. |
| Key words: Xinnaoning tablets cerebral ischemia lactic acid LDH, ATPase activity |
