| 引用本文: | 王海荣,张兰桐.HPLC-MS/MS在药物代谢和药动学筛选中的应用[J].中国现代应用药学,2014,31(5):634-638. |
| WANG Hairong,ZHANG Lantong.HPLC-MS/MS in Drug Metabolism and Pharmacokinetic Screening[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(5):634-638. |
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| 摘要: |
| 目的 本综述简要介绍先导化合物优化阶段，HPLC-MS/MS应用于药物代谢和药动学(DMPK)中筛选一系列新的化学实体。方法 查阅有关文献，进行分析总结。结果 HPLC-MS/MS在支持体内或体外试验以及定性分析，如代谢物鉴定等方面均有广泛应用。结论 液质联用已成为DMPK筛选强有力且必不可少的分析手段。 |
| 关键词: 药物代谢和药动学 药物开发 高效液相色谱-质谱联用 药动学 |
| DOI： |
| 分类号:R944 |
| 基金项目:国家科技重大专项课题(2009ZX09313-003，2009ZX09102-140) |
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| HPLC-MS/MS in Drug Metabolism and Pharmacokinetic Screening |
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WANG Hairong1,2, ZHANG Lantong1
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1.School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China;2.Shijiazhuang Yiling Pharmaceutical Co., Ltd., Shijiazhuang 050035, China
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| Abstract: |
| OBJECTIVE This review summarizes application of high performance liquid chromatography coupled to a tandem mass spectrometer(HPLC-MS/MS) methodology in drug metabolism and pharmacokinetic screens for a series of new chemical entities during the lead optimization stages. METHODS Relevant literatures were consulted, analysis and summary were made. RESULTS The HPLC-MS/MS methods were widely used to support both in vitro and in vivo experiments and for the qualitative assays, such as metabolite identification. CONCLUSION HPLC-MS/MS is a powerful and indispensable analytical tool in drug metabolism and pharmacokinetic screening. |
| Key words: DMPK drug development HPLC-MS/MS pharmacokinetics |
