| 引用本文: | 李煜,金瓯,郑国钢,洪利娅.氨基酸分析仪法(过甲酸氧化)测定复方氨基酸注射液(18AA)中胱氨酸的含量[J].中国现代应用药学,2014,31(7):871-874. |
| LI Yu,JIN Ou,ZHENG Guogang,HONG Liya.Determination of the Content of Cystine in Compound Amino Acids Injection (18AA) with Performic Acid Oxidation by Amino Acid Analyzer[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(7):871-874. |
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| 摘要: |
| 目的 建立测定复方氨基酸注射液(18AA)中胱氨酸的含量方法。方法 采用柱前衍生-氨基酸分析仪测定。结果 胱氨酸在20.46~184.10 μg·mL-1内呈良好的线性关系(r=0.999 8)，平均回收率为100.8%，RSD(n=9)为0.91%。结论 本法专属性强、准确、快速、灵敏，可用于测定复方氨基酸注射液中胱氨酸的含量。 |
| 关键词: 复方氨基酸注射液 胱氨酸 含量测定 氨基酸分析仪 |
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| Determination of the Content of Cystine in Compound Amino Acids Injection (18AA) with Performic Acid Oxidation by Amino Acid Analyzer |
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LI Yu, JIN Ou, ZHENG Guogang, HONG Liya
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Zhejiang Institute for Food and Drug Control, Hangzhou 310004, China
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| Abstract: |
| OBJECTIVEE To establish a method to determine the content of cystine in Compound Amino Acids injection (18AA). METHODS The pre-column derivation and amino acid analyzer were adopted. RESULTS This method showed that the concentration-response relationship was linear over the range of 20.46-184.10 μg·mL-1(r=0.999 8). The average recovery was 100.8% (RSD=0.91%, n=9). CONCLUSION The method is specific, accurate, rapid, sensitive and suitable for the determination of content of cystine in Compound Amino Acids injection (18AA). |
| Key words: Compound Amino Acids injection (18AA) cystine content determination amino acid analyzer |
