| 引用本文: | 王丽彬,孟玲.应用环介导等温扩增法进行卡马西平安全用药监测[J].中国现代应用药学,2014,31(5):574-577. |
| WANG Libin,MENG Ling.Monitoring the Safety of Carbamazepine by Loop Mediated Isothermal Amplification[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(5):574-577. |
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| 摘要: |
| 目的 建立环介导等温扩增法(loop-mediated isothermal amplification，LAMP)检测卡马西平引起严重皮肤损害的标志基因HLA-B*1502的技术平台。方法 对所收集的病例，分别应用LAMP和金标准SSP-PCR进行标志基因HLA-B*1502的检测，验证LAMP方法的准确性。结果 LAMP和SSP-PCR检测38例对照组和2例药疹组患者基因型结果完全一致。结论 LAMP操作简单快捷，且准确可靠，可以在临床上广泛推广，提高卡马西平安全用药水平。 |
| 关键词: 卡马西平 SJS/TEN HLA-B*1502 环介导等温扩增法 引物序列特异性PCR |
| DOI： |
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| 基金项目:南京市2012年度科技发展计划(药学项目)(2012YX006) |
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| Monitoring the Safety of Carbamazepine by Loop Mediated Isothermal Amplification |
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WANG Libin, MENG Ling
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Department of Pharmacy, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
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| Abstract: |
| OBJECTIVE To establish a technology platform for testing the marker gene HLA-B*1502 of severe epispasis induced by carbamazepine using the loop-mediated isothermal amplification(LAMP). METHODS The marker gene HLA-B* 1502 was tested by LAMP method and the SSP-PCR method, respectively. And the accuracy of the LAMP method was verified. RESULTS The genotype results of control group and drug eruption group tested with LAMP method were consistent with those using SSP-PCR method. CONCLUSION LAMP method is simple and quick to be operated. It’s reliable and accurate, and can be widely applied in clinic. |
| Key words: carbamazepine SJS/TEN HLA-B*1502 LAMP SSP-PCR |
