| 引用本文: | 张琳,李晓誉,徐世芳,陈峰阳,叶益萍.双波长HPLC测定青阳参中青阳参苷元和告达庭的含量[J].中国现代应用药学,2014,31(3):317-319. |
| ZHANG Lin,LI Xiaoyu,XU Shifang,CHEN Fengyang,YE Yiping.Determination of Cynanchogenin and Caudatin in Cynanchum Otophyllum Scheid by HPLC under Double UV Wavelengths[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(3):317-319. |
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| 摘要: |
| 目的 建立采用双波长HPLC同时测定青阳参中青阳参苷元和告达庭含量的方法。方法 采用高效液相色谱法，ZORBAX SB-C18(4.6 mm×150 mm，5 μm)色谱柱，柱温40 ℃，流动相为乙腈-水，梯度洗脱，流速为1.0 mL·min-1，检测波长为220，260 nm。结果 青阳参苷元和告达庭均在2~64 μg·mL-1内呈良好的线性关系，r均为1；平均回收率为100.1%和97.9%，RSD值分别为1.34%和1.37%(n=6)。结论 该方法快捷简单，精密可靠，重复性好，可为青阳参及其制剂的质量控制提供参考。 |
| 关键词: 高效液相色谱法 青阳参 青阳参苷元 告达庭 |
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| 基金项目:浙江省科技计划项目(2011F20013)；国家自然科学基金(21272213)；浙江省医学重点学科群(XKQ-010-001) |
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| Determination of Cynanchogenin and Caudatin in Cynanchum Otophyllum Scheid by HPLC under Double UV Wavelengths |
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ZHANG Lin, LI Xiaoyu, XU Shifang, CHEN Fengyang, YE Yiping
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Zhejiang Academy of Medical Science, Hangzhou 310013, China
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| Abstract: |
| OBJECTIVE To estabish an HPLC method under double UV wavelengths for determination of cynanchogenin and caudatin in Cynanchum otophyllum Scheid. METHODS ZORBAX SB-C18 column (4.6 mm×150 mm, 5 μm) was used and the temperature of column was 40 ℃. The mobile phase consisted of methanol-acetonitrile with gradient elution. The flow rate was 1.0 mL·min-1. The detection wavelength was set at 220 and 260 nm. RESULTS The cynanchogenin and caudatin had a good linearity relationship in 2 μg·mL-1, with r=1. The average recoveries were 100.1% and 97.9% with RSD of 1.34% and 1.37%(n=6). CONCLUSION The method is simple, rapid, accurate and provides the reference for the quality identification of Cynanchum otophyllum Scheid and its preparation. |
| Key words: HPLC Cynanchum otophyllum Scheid cynanchogenin caudatin |
