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引用本文:刘慧璐,冯建勇,王增尚,吴晨,张末初,周胜,刘江云,郝丽莉.高色价栀子黄的精制工艺研究[J].中国现代应用药学,2013,30(12):1315-1319.
LIU Huilu,FENG Jianyong,WANG Zengshang,WU Chen,ZHANG Mochu,ZHOU Sheng,LIU Jiangyun,HAO Lili.Preparative Separation of Crocins with High Color Value from Gardenia Fruits[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(12):1315-1319.
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高色价栀子黄的精制工艺研究
刘慧璐, 冯建勇, 王增尚, 吴晨, 张末初, 周胜, 刘江云, 郝丽莉
苏州大学药学院,江苏 苏州 215123
摘要:
目的 对栀子黄进行进一步分离制备,以获得高色价的西红花总苷及其中主要单体成分。方法 采用中压反相柱层析技术,对栀子黄进行进一步分离制备,采用液相色谱检测西红花苷-1含量,采用紫外可见光谱检测色价及其吸光度比值。结果 制备工艺可有效去除绿原酸等杂质,精制后的西红花总苷样品中西红花苷-1含量为60.8%,色价高达756;并可分离获得西红花苷-1(1)、西红花苷-2(2)、西红花苷-3(3)等化合物。结论 该制备方法简便、高效、成本较低,可为栀子黄的精制及产业化生产提供参考。
关键词:  栀子  栀子黄  西红花苷  分离  反相柱层析
DOI:
分类号:R284.1;R917.101
基金项目:苏州大学大学生创新创业训练计划项目(2012xj065)
Preparative Separation of Crocins with High Color Value from Gardenia Fruits
LIU Huilu, FENG Jianyong, WANG Zengshang, WU Chen, ZHANG Mochu, ZHOU Sheng, LIU Jiangyun, HAO Lili
College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China
Abstract:
OBJECTIVE To establish an efficient separation process to afford gardenia yellow and crocins with high color value. METHODS The process was conducted using reversed-phase medium pressure liquid chromatography. A high pressure liquid chromatography - photodiode array method was applied for qualitative and quantitative analysis of crocin-1. Moreover, the color value was also evaluated by UV method. RESULTS After the reversed-phase separation, the content of crocin-1 in refined product reached 60.8%, with the color value to be 756. Crocin-1(1), crocin-2(2) and crocin-3(3) could be separated either with similar procedure. CONCLUSION The process developed is highly efficient, low-cost and compact, which affords a potential approach for industrial production of crocins with improved quality.
Key words:  Gardenia jasminoides  gardenia yellow  crocin  separation  reversed-phase chromatography
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