| 引用本文: | 郑利,陈丹,曾令军,蔡韦炜,连赟芳,范世明.UPLC-MS测定不同产地薏苡仁中甘油三油酸酯的含量[J].中国现代应用药学,2014,31(2):200-204. |
| ZHENG Li,CHEN Dan,ZENG Lingjun,CAI Weiwei,LIAN Yunfang,FAN Shiming.Determination of Triolein Content in Coicis Semen from Different Regions by UPLC-MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(2):200-204. |
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| 摘要: |
| 目的 建立测定甘油三油酸酯的UPLC-MS，并对23个不同产地的薏苡仁中甘油三油酸酯含量进行测定。方法 使用Waters Quattro Micro液质联用仪，色谱柱为Acquity UPLC BEH C18柱(2.1 mm×100 mm，1.7 μm)；流动相(A)为甲醇-乙腈-氨水(19∶19∶2)调至pH 3的0.1%甲酸溶液，流动相(B)为异丙醇，A-B(55∶45)；柱温45 ℃；流速0.3 mL·min-1；ESI+，多反应监测模式，监测离子为m/z 902.8→m/z 603.2。结果 标准曲线为Y=4.233×103X+ 9.172×102(r=0.999 5)，甘油三油酸酯在0.142~25.56 μg·mL-1内具有良好的线性关系；平均回收率为97.21%，RSD为1.44%(n=9)；不同产地来源薏苡仁中甘油三油酸酯含量存在差异。结论 本方法灵敏、准确、重复性好，可用于薏苡仁及薏苡仁油的质量控制。 |
| 关键词: UPLC-MS 薏苡仁 甘油三油酸酯 质量控制 |
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| 基金项目:福建省科技计划项目(2010Y2004) |
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| Determination of Triolein Content in Coicis Semen from Different Regions by UPLC-MS |
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ZHENG Li, CHEN Dan, ZENG Lingjun, CAI Weiwei, LIAN Yunfang, FAN Shiming
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Department of Pharmacy, Fujian University of TCM, Fuzhou 350122, China
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| Abstract: |
| OBJECTIVE To establish a ultra-high performance liquid chromatography with mass(UPLC-MS) method for determination of triolein content, and determine triolein content in Coicis Semen from 23 different regions. METHODS Using Waters Quattro Micro with mass, the column was Acquity UPLC BEH C18(2.1 mm×100 mm, 1.7 μm); the mobile phase was made up that A phase contained methanol, acetonitrile and 0.1% formic acid solution with ammonia to pH 3 in the proportion for 19∶19∶2, and B phase was isopropyl alcohol, the proportion of A phase and B phase was 55∶45; column temperature was 45 ℃; the flow rate was 0.3 mL·min-1. Detection in ESI using positive ionization and multiple reactions monitoring mode(MRM) was applied to determine positive ions m/z 902.8→m/z 603.2. RESULTS The standard curve was Y=4.233×103X+9.172×102(r=0.999 5), the tirolein in linear range of 0.142-25.56 μg·mL-1 had a good linear relationship; the average recovery was 97.21%, RSD was 1.44%(n=9); and triolein content in Coicis Semen from different regions was different. CONCLUSION The established UPLC-MS method on determination of triolein content in Coicis Semen is sensitive, rapid, accurate, reproducible and can be used for the quality control of Coicis Semen. |
| Key words: UPLC-MS Coicis Semen triolein quality control |
