| 引用本文: | 赵韶华,高淑丽,张利康,张晨光,高学东,刘敏彦.UPLC同时测定百灵安神片中七种活性成分含量[J].中国现代应用药学,2013,30(12):1331-1333. |
| ZHAO Shaohua,GAO Shuli,ZHANG Likang,ZHANG Chenguang,GAO Xuedong,LIU Minyan.Simultaneous Determination of Seven Active Compounds in Bailing Anshen Tablets by UPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(12):1331-1333. |
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| 摘要: |
| 目的 建立UPLC同时测定百灵安神片中绿原酸、芒果苷、二苯乙烯苷、斯皮诺素、木犀草苷、槲皮苷、3，5-二咖啡酰奎宁酸含量的方法。方法 采用Waters Acquity UPLC BEH C18(2.1 mm×100 mm，1.7 μm)色谱柱；流动相：乙腈-0.1%磷酸水，梯度洗脱；流速：0.4 mL·min-1；检测波长：340 nm。结果 绿原酸、芒果苷、二苯乙烯苷、斯皮诺素、木犀草苷、槲皮苷、3，5-二咖啡酰奎宁酸在检测范围内线性良好，r均>0.999 8，方法回收率分别96.3%，98.2%，100.5%，99.3%，97.7%，97.0%，96.4%。结论 本方法准确可靠快捷，为全面控制百灵安神片质量提供了一种测定方法。 |
| 关键词: 超高效液相色谱法 百灵安神片 质量控制 |
| DOI： |
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| 基金项目:“重大新药创制”科技重大专项(2011ZX09401-306) |
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| Simultaneous Determination of Seven Active Compounds in Bailing Anshen Tablets by UPLC |
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ZHAO Shaohua1, GAO Shuli2, ZHANG Likang1, ZHANG Chenguang1, GAO Xuedong1, LIU Minyan1
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1.Shijiazhuang Yiling Pharmaceutical Co., Ltd., Shijiazhuang 050035, China;2.The Fourth Hospital of Shijiazhuang, Shijiazhuang 050017, China
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| Abstract: |
| OBJECTIVE To established a UPLC method for determination of seven active compounds in Bailing Anshen tablets, including chlorogenic acid, mangiferin, tetrahydroxystilbene, spinosin, cynaroside, quercitrin, and 3,5-dicaffeoylquinic. METHODS The chromatographic separation performed on a C18 column and with gradient elution of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 0.4 mL·min-1. The detected wavelength was set at 340 nm. RESULTS The seven analytes showed good linearity (r>0.999 8) within the test ranges. The average recoveries were 96.3%, 98.2%, 100.5%, 99.3 %, 97.7%, 97.0%, 96.4%, respectively. CONCLUSION The analytical method is simple, rapid, specific, which is useful for quality control of Bailing Anshen tablets. |
| Key words: UPLC Bailing Anshen tablets quality control |
